KitforusewithRocheLightCycler480Real-TimePCRSystem,includes100barcodedclearshell/whitewellPCRplates(4packsofHSR9905)and100Microseal®"C"OpticalSeals(MSC1001)Description:
ThisHard-Shell96-Well480PCRPlatesKitcontains100platesand100Microseal"C"OpticalSeals(MSC1001).
Microseal"C"OpticalSealsareopticallyclearadhesivefilmsdesignedforopticalassays,suchasthoseperformedinreal-timePCR.Theycanbeusedformicroplateswithraisedwellrims.
KeyFeatures
- FilmsprovideatightsealforPCRandqPCR
- Pressure-sensitiveadhesivestickstotheplate,notyourgloves
- FreefromDNase,RNase,andhumanDNAcontaminants
Hard-Shell96-well480PCRPlatesareoptimizedtoworkontheRocheLightCycler480real-timePCRsystem.Hard-ShellPlatesarespecificallydesignedtowithstandthestressesofheatsealing,thermalcycling,androbotichandling.Thepatented*two-componentmicroplatedesignprovidessuperiorstABIlityandflatness,allowingprecisepositioningforautomation.Thelow-profilewellsareoptimizedforlow-volumereactionsandfastPCR.
KeyFeaturesandBenefits
- Polycarbonateshell—preventsplatewarpingwhenthermalcyclingintheRocheLightCycler480
- Extremelyuniformwells—reducewell-to-wellvariabilityinopticalassaysperformedinreal-timePCR
- Blacklettering—facilitateswellidentification
- Rigidshell—idealforheatsealingmethodswithinstrumentssuchasthePX1™PCRplatesealer

Notdrawntoscale.
Alldimensionsinmm,unlessotherwisenoted.
| MicroplateDimensions | Hard-Shell®96-Well480PCRPlates(HSR-99xx) |
| Lengthatbaseplane | 127.76mm |
| Widthatbaseplane | 85.48mm |
| Heightoverall | 15.51mm |
| Welldepth | 14.41mm |
| Welldiameteratopening | 5.46mm |
| Welldiameteratbottomofconicalsection | 3.00mm |
| Wellvolume | 200μl |
| Wellspacing | 9.00mm |
| Wellangle | 17.0° |
| Welloffset | |
| LeftedgetowellA1 | 14.38mm |
| TopedgetowellA1 | 11.24mm |
| CenterofA1tocenterofA12 | 99.00mm |
| CenterofA1tocenterofH1 | 63.00mm |
AdditionalDetails
- White-welloptionforincreasedfluorescentsignalstrength
- Bar-codedonrowAsideforconvenientdatabasetracking
- FootprintandwellspacingmatchANSI/SBSstandarddimensions
- Reactionvolumesof5–125µl(200µlmaximum)
- ProcesssamplingandtestingtoensurethatplatesarefreeofDNase,RNase,andhumanDNA
CompatIBLePCRInstrument
- RocheLightCycler480real-timePCRsystem
*Hard-ShellplatesarecoveredbyoneormoreofthefollowingU.S.patentsortheirforeigncounterpartsownedbyEppendorfAG:U.S.PatentNumbers7,347,977;6,340,589;and6,528,302.
Specifications:
| Well/tubeformat | 96-well |
| Well/tubeprofile | Low |
| Skirtformat | Full-skirted |
| BarcodedPCRplate | Yes |
| CompatiblePCRinstrument | RocheLightCycler480 |
| Well/tubecolor | White |