• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： NEB >>> NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/250 preps
    NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/250 preps
    • NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/250 preps

    NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/250 preps

    價格: ￥5460.00 市場價: 9100.00
    打印產品信息
    貨號: T1030S-250preps
    品牌: NEB
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即購買 加入購物車

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題

      • Description:

        ?
        TheMonarchPCR&DNACleanupKitrapidlyandreliablypurifiesupto5μgofconcentrated,high-qualityDNAfromPCRandotherenzymaticreactions.Thekitutilizesabind/wash/eluteworkflowwithminimalincubationandspintimes.Thecolumnsensurezerobufferretentionandnocarryoverofcontaminants,enablingelutionofsampleinvolumesaslowas6μl.Thebuffersprovidedhavebeenoptimized,anddonotrequiremonitoringofpH.ElutedDNAisreadyforuseinrestrictiondigests,DNAsequencing,ligationandotherenzymaticmanipulations.DesignedwithsustainABIlityinmind,MonarchkitsusesignificantlylessplasticandresponsIBLy-sourced,recyclablepackaging.TheprotocolcanalsobemodifiedtoenablethepurificationofsmallerDNAfragments,includingoligonucleotides.

        Viewourvideosonprotocols,tips,andrecyclingMonarch.

        ?
        APPLICATIONS
        PCRcleanupDNAfromPCRreactionscanbepurifiedafteramplificationtoremovepolymerases,primers,detergents,dNTPs,etc.
        EnzymaticreactioncleanupModifyingenzymessuchasligases,kinases,nucleases,phosphatasesareefficientlyremovedallowingefficientdesaltingandconcentrationoftheDNAsample.
        CDNAcleanupDNA/RNAcomplexescanbepurifiedpost-reversetranscription/amplificationtoenableremovaloftheRTandpolymeraseaswellasnucleotides.
        LabelingcleanupUnincorporatedrADIolabeledorfluorescentlylabelednucleotidescanberemovedfromtheDNAsubstrate
        PlasmidcleanupPlasmidprepsfromunknownsourcesmaycontaininhibitorsandunwantedcontaminants.Purificationandconcentrationcanbeeasilyachievedusingthiskit.
        OligonucleotidecleanupssDNAoligonucleotides(≥18nt)anddsDNAfragments(≥?15bp)canbepurifiedusingtheOligonucleotideCleanupProtocol.


        MonarchDNACleanupColumnDesign
        Monarch DNA Cleanup Column Design

        Monarchcolumnsaredesignedforperformance
        Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
        Monarchcolumnsaredesignedwithoutafrit,whicheliminatesbufferretentionandtheriskofcarryovercontamination,providingfast,worry-freeDNApurification.

        ?Advantages:
        • Eluteinaslittleas6μl
        • Preventbufferretentionandsaltcarry-overwithoptimizedcolumndesign
        • Savetimewithfast,user-friendlyprotocols
        • NoneedtomonitorpH
        • Buffersandcolumnsavailableseparately
        • Significantlylessplasticusedwhencomparedwithotherkits?
        • Responsibly-sourcedandrecyclablepackaging?

        Specifications

        DNASampleType:DNAfromPCRandotherenzymaticreactions(e.g.,restrictiondigests,kinasereactions,ligations).*
        BindingCapacity:upto5μg
        DNASizeRange:~50bpto25kb**
        TypicalRecovery:DNA(50bpto10kb):70–90%/?DNA(11–23kb):50–70%
        ElutionVolume:≥6μl
        Purity:A260/280>1.8andA260/230>1.8
        ProtocolTime:5minutesofspinandincubationtime
        CompatibleDownstream
        Applications:        		ligation,restrictiondigestion,labelingandotherenzymatic
        manipulations,libraryconstructionandDNAsequencing.

        *ssDNAordsDNAoligonucleotidesfromenzymaticreactionscanalsobepurifiedusingtheOligonucleotideCleanupProtocol.
        **DNA≥15bpto25kb(dsDNA)andDNA≥18ntto10kb(ssDNA)canalsobepurifiedusingtheOligonucleotideCleanupProtocol.
        ?

        MonarchPCR&DNACleanupKit(5μg)Protocol?
        Monarch PCR & DNA Cleanup Kit (5 μg) Protocol

        MonarchPCR&DNACleanupKit(5μg)performsequivalentlytotheleadingsupplier
        Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplier. Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq? Quick-Load? 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF? (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material.
        Prepswereperformedaccordingtorecommendedprotocols.1μgofa3kbDNAfragmentwasincubatedwith1μMprimersandOneTaq?Quick-Load?2XMasterMix(NEB#M0486).DNAwaselutedin20μl(NEB)and40μl(Qiagen)ElutionBuffer.Halfofthetotalelutionvolumewasdigestedwith5unitsofDraIII-HF?(NEB#R3510).Thedigestandtheunusedportionoftheelutionwereresolvedona1%w/vagarosegelalongwitharepresentativesampleofthestartingmaterial.?
        MonarchPCR&DNACleanupKit(5μg)removeslowmolecularweightprimersfromdsDNAsamples
        Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II.
        Threeindependentamplicons(267bp,520bp,1003bp)werespikedwithtwooligonucleotides(16-mer,24-mer)toafinalconcentrationof1μM.HalfofeachmixwaspurifiedwiththeMonarchPCR&DNACleanupKit(5μg)followingtheincludedprotocol.Equivalentfractionsoftheoriginalmixtureandtheelutedmaterialwereresolvedona20%TBEacrylamidegelat100VforonehourandstainedwithSYBRGreenII.

        LearnMoreAboutMonarch
        • NEBMonarch.com
        • Optimizeyourresultswithouruniquecolumndesign
        • EnhanceyourDNApurificationexperience
        • FeelgoodaboutchoosingMonarch
        • ChooseMonarchkitsforpurevalue

        KitComponents

        Thefollowingreagentsaresuppliedwiththisproduct:

        Storeat(°C)Concentration
        Monarch®DNACleanupBindingBuffer251X
        Monarch®DNAWashBuffer255X
        Monarch®DNAElutionBuffer251X
        Monarch®DNACleanupColumns(5μg)25

        Notes:

        Thekitshouldbestoredatroomtemperature.Alwayskeepbufferbottlestightlyclosedandkeepcolumnssealedintheenclosedzip-lockbag.Forinformationregardingthecompositionofbuffers,pleaseconsulttheSafetyDataSheets.Properlaboratorysafetypracticesshouldbeemployed,includingtheuseoflabcoats,glovesandeyeprotection.
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： ImmunoStar中國 | PelFreez-Bio價格 | KawamuraChemical華東代理 | maxvisionbio官網 | ezbiosystems代理 | 螞蟻淘 | 細胞培養瓶

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频