Immunofluorescence (1 - 4 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:500)Western Blot (1:100 - 1:250)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 4°C for short term storage. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Transfected lysate)
Western Blot analysis with AFP polyclonal antibody (Cat # PAB30577).Lane 1: Negative control (vector only transfected HEK293T lysate)Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with AFP polyclonal antibody (Cat # PAB30577) shows extra cellular positivity.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with AFP polyclonal antibody (Cat # PAB30577) shows positivity in cytoplasm. Antibody staining is shown in green.
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
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