Rabbit polyclonal antibody raised against synthetic peptide of SARS-CoV nonstructural protein 13 (nsp13).
Immunogen:
A synthetic peptide (conjugated with BSA) corresponding to amino acids 584-601 at C-terminus of SARS-CoV nsp13.
Host:
Rabbit
Reactivity:
SARS-CoV
Specificity:
This antibody is directed against SARS-Coronavirus nsp13 protein.
Form:
Liquid
Purity:
The product is neat antiserum.
Recommend Usage:
Immunoelectron Microscopy (1:300)Immunofluorescence (1:800)Immunoprecipitation (1:60)Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In antiserum (0.01% sodium azide).
Storage Instruction:
Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage.Aliquot to avoid repeated freezing and thawing.Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins.Prentice E, McAuliffe J, Lu X, Subbarao K, Denison MR.J Virol. 2004 Sep;78(18):9977-86.
2.
Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase.Ivanov KA, Thiel V, Dobbe JC, van der Meer Y, Snijder EJ, Ziebuhr J.J Virol. 2004 Jun;78(11):5619-32.
3.
Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LL, Guan Y, Rozanov M, Spaan WJ, Gorbalenya AE.J Mol Biol. 2003 Aug 29;331(5):991-1004.
Applications
Western Blot
Immunofluorescence
Immunofluorescence Microscopy using SARS-CoV nsp13 polyclonal antibody (Cat # PAB11370), 6 hrs post infection Vero-E6 cells.Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
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