The antiserum was produced against synthesized phospho-peptide derived from human Survivin around the phosphorylation site of Thr34.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Survivin (Phospho-Thr34) Antibody detects endogenous levels of BIRC5 only when phosphorylated at Thr34.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BIRC5
Database Links
Gene ID: 332 Swiss-Prot #: O15392
OMIM #: 603352 Unigene #: Hs.514527
Target Modification
Phospho
Modification Sites
H:T34, M:T34, R:T34
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Survivin (Phospho-Thr34) Antibody
Image 2
Western blot analysis of lysates from HT29 cells treated with EGF 100ug/ml 30', using Survivin (Phospho-Thr34) Antibody.