The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody
Synonyms
40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human S6 Ribosomal Protein around the phosphorylation site of Ser235+Ser236.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody detects endogenous levels of RPS6 only when phosphorylated at Ser235+Ser236.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6
Database Links
Gene ID: 6194 Swiss-Prot #: P62753
OMIM #: 180460 Unigene #: Hs.408073
Target Modification
Phospho
Modification Sites
H:S235+S236, M:S235+S236, R:S235+S236
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody
Image 2
Western blot analysis of Jurkat treated with TNF and NIH/3T3 cells treated with PMA, using S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody.