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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody |
| Synonyms | Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1; RARA; NR1B1 |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human Retinoic Acid Receptor alpha around the phosphorylation site of Ser77. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
| Specificity | Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody detects endogenous levels of RARA only when phosphorylated at Ser77. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | RARA |
| Database Links |
Gene ID: 5914 Swiss-Prot #: P10276 OMIM #: 180240 Unigene #: Hs.654583 |
| Target Modification | Phospho |
| Modification Sites | H:S77, M:S77, R:S74 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoic Acid Receptor α (Phospho-Ser77) Antibody |
| Image 2 |
 Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. |
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