Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | PDGFRbeta(Phospho-Tyr740)Antibody |
Synonyms | CD140b;kinasePDGFR-beta;PDGF-R-beta;PDGFR;PDGFR-beta;PGFRB;platelet-derivedgrowthfactorreceptor,betapolypeptide |
Immunogen | Theantiserumwasproducedagainstsynthesizedphospho-peptidederivedfromhumanPDGFRbetaaroundthephosphorylationsiteofTyr740. |
ImmunogenRange | |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:10000 |
Specificity | PDGFRbeta(Phospho-Tyr740)AntibodydetectsendogenouslevelsofPDGFRBonlywhenphosphorylatedatTyr740. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(Phospho-withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | PDGFRB |
DatabaseLinks | GeneID: 5159 Swiss-Prot#: P09619 OMIM#: 131440/173410 Unigene#: Hs.509067 |
TargetModification | Phospho |
ModificationSites | H:Y740,M:Y739,R:Y739 |
ApplicationImages |
Image1 | Enzyme-LinkedImmunosorbentAssay(Phospho-ELISA)forImmunogenPhosphopeptide(Phospho-left)andNon-Phosphopeptide(Phospho-right),usingPDGFRβ(Phospho-Tyr740)Antibody |
Image2 | WesternblotanalysisoflysatesfromHepG2cellstreatedwithEGF200ng/ml30",usingPDGFRbeta(Phospho-Tyr740)Antibody. |