The antiserum was produced against synthesized phospho-peptide derived from human MAP2K1 around the phosphorylation site of Thr386.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
MAP2K1 (Phospho-Thr386) Antibody detects endogenous levels of MAP2K1 only when phosphorylated at Thr386.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K1
Database Links
Gene ID: 5604 Swiss-Prot #: Q02750
OMIM #: 115150/176872 Unigene #: Hs.145442
Target Modification
Phospho
Modification Sites
H:T386, M:T386, R:T386
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K1 (Phospho-Thr386) Antibody
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with EGF, RAW264.7 cells treated with UV, and A549 cells treated with UV, using MAP2K1 (Phospho-Thr386) Antibody.