Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | KIT(Phospho-Tyr936)Antibody |
Synonyms | SCFR;Proto-oncogenetyrosine-proteinkinaseKit;c-kit;CD117antigen |
Immunogen | Theantiserumwasproducedagainstsynthesizedphospho-peptidederivedfromhumanKITaroundthephosphorylationsiteofTyr936. |
ImmunogenRange | |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:10000 |
Specificity | KIT(Phospho-Tyr936)AntibodydetectsendogenouslevelsofKITonlywhenphosphorylatedatTyr936. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(Phospho-withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | KIT |
DatabaseLinks | GeneID: 3815 Swiss-Prot#: P10721 OMIM#: 164920/172800/273300/606764 Unigene#: Hs.479754 |
TargetModification | Phospho |
ModificationSites | H:Y936,M:Y934 |
ApplicationImages |
Image1 | Enzyme-LinkedImmunosorbentAssay(Phospho-ELISA)forImmunogenPhosphopeptide(Phospho-left)andNon-Phosphopeptide(Phospho-right),usingKIT(Phospho-Tyr936)Antibody |
Image2 | WesternblotanalysisoflysatesfromHepG2cellstreatedwithEGF200ng/ml30",usingKIT(Phospho-Tyr936)Antibody. |