The antiserum was produced against synthesized phospho-peptide derived from human IKK-alpha around the phosphorylation site of Ser176 and IKK-beta around the phosphorylation site of Ser177.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
IKK-alpha (Phospho-Ser176)/IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of CHUK/IKBKB only when IKK-alpha phosphorylated at Ser176 and IKK-beta phosphorylated at Ser177.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-α (Phospho-Ser176) /IKK-β (Phospho-Ser177) Antibody
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody.