The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
HS1 (Phospho-Tyr397) Antibody
Synonyms
HCLS1; Hematopoietic cell- specific LYN substrate 1; Hematopoietic lineage cell specific protein; LCKBP1
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human HS1 around the phosphorylation site of Tyr397.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
HS1 (Phospho-Tyr397) Antibody detects endogenous levels of HCLS1 only when phosphorylated at Tyr397.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HCLS1
Database Links
Gene ID: 3059 Swiss-Prot #: P14317
OMIM #: 601306 Unigene #: Hs.14601
Target Modification
Phospho
Modification Sites
H:Y397
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HS1 (Phospho-Tyr397) Antibody
Image 2
Western blot analysis of Jurkat cells treated with TNF, using HS1 (Phospho-Tyr397) Antibody.