The antiserum was produced against synthesized phospho-peptide derived from human BCL2 around the phosphorylation site of Ser70.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
BCL2 (Phospho-Ser70) Antibody detects endogenous levels of BCL2 only when phosphorylated at Ser70.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCL2
Database Links
Gene ID: 596 Swiss-Prot #: P10415
OMIM #: 151430 Unigene #: Hs.150749
Target Modification
Phospho
Modification Sites
M:S70, R:S70
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BCL2 (Phospho-Ser70) Antibody
Image 2
Western blot analysis of lysates from 293, Jurkat and NIH/3T3 cells treated with Piclitaxel, using BCL2 (Phospho-Ser70) Antibody.