The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
AKT1/3 (Phospho-Tyr437/434) Antibody
Synonyms
RAC-PK-alpha; Protein kinase B; PKB; C-AKT
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human AKT1/3 around the phosphorylation site of Tyr437/Tyr434.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
AKT1/3 (Phospho-Tyr437/Tyr434) Antibody detects endogenous levels of AKT1/AKT3 only when phosphorylated at Tyr437/Tyr434.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AKT1/3 (Phospho-Tyr437/434) Antibody
Image 2
Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15', using AKT1/3 (Phospho-Tyr437/434) Antibody.