The antiserum was produced against synthesized phospho-peptide derived from human PP1alpha around the phosphorylation site of Thr320.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
PP1alpha (Phospho-Thr320) antibody detects endogenous levels of PP1alpha only when phosphorylated at Thr320.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PPP1CA
Database Links
Gene ID: 5499 Swiss-Prot #: P62136
OMIM #: 176875 Unigene #: Hs.183994
Target Modification
Phospho
Modification Sites
H:T320, M:T320, R:T320
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PP1α (Phospho-Thr320) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells, using p-PP1α (Phospho-Thr320) antibody.