The antiserum was produced against synthesized phospho-peptide derived from human PI 3-kinase p85beta around the phosphorylation site of Tyr464.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
PI 3-kinase p85beta (Phospho-Tyr464) antibody detects endogenous levels of PI 3-kinase p85beta only when phosphorylated at Tyr464.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PIK3R2
Database Links
Gene ID: 5296 Swiss-Prot #: O00459
OMIM #: 603157 Unigene #: Hs.371344
Target Modification
Phospho
Modification Sites
H:Y464, M:Y458, R:Y458
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PI 3-kinase p85β (Phospho-Tyr464) Antibody
Image 2
Western blot analysis of lysate from Jurkat cells, using phospho-PI 3-kinase p85β (Phospho-Tyr464) antibody.