The antiserum was produced against synthesized phospho-peptide derived from human Flt-3 around the phosphorylation site of Tyr591.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Flt-3 (Phospho-Tyr591) antibody detects endogenous levels of Flt-3 only when phosphorylated at Tyr591.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FLT3
Database Links
Gene ID: 2322 Swiss-Prot #: P36888
OMIM #: 136351 Unigene #: Hs.507590
Target Modification
Phospho
Modification Sites
H:Y591, M:Y592
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Flt-3 (Phospho-Tyr591) Antibody
Image 3
Immunohistochemistry analysis of phospho-FLT3 (Phospho-Tyr591) antibody in paraffin-embedded human lung adenocarcinoma tissue.