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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | DDX3 (Phospho-Tyr69) Antibody |
| Synonyms | ATP-dependent RNA helicase DDX3X; DEAD box protein 3 X-chromosomal; DEAD box X isoform; Helicase-like protein 2; HLP2; DDX3X; DBX; DDX3; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3 Y-chromosomal; DDX3Y; DBY |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human DDX3 around the phosphorylation site of Tyr69. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:10000 |
| Specificity | DDX3 (Phospho-Tyr69) antibody detects endogenous levels of DDX3 only when phosphorylated at Tyr69. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | DDX3X/DDX3Y |
| Database Links |
Gene ID: 1654/8653 Swiss-Prot #: O00571/O15523 OMIM #: 300160/400010 Unigene #: Hs.380774/Hs.99120 |
| Target Modification | Phospho |
| Modification Sites | H:Y69, M:Y69 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DDX3 (Phospho-Tyr69) Antibody |
| Image 2 |
 Western blot analysis of lysates from HepG2 cells, using phospho-DDX3 (Phospho-Tyr69) antibody. |
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