The antiserum was produced against synthesized phospho-peptide derived from human ALK around the phosphorylation site of Tyr1586.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
ALK (Phospho-Tyr1586) antibody detects endogenous levels of ALK only when phosphorylated at Tyr1586.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ALK
Database Links
Gene ID: 238 Swiss-Prot #: Q9UM73
OMIM #: 105590 Unigene #: Hs.654469
Target Modification
Phospho
Modification Sites
H:Y1586, M:Y1592
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ALK (Phospho-Tyr1586) Antibody
Image 3
Immunohistochemistry analysis of phospho-ALK (Phospho-Tyr1586) antibody in paraffin-embedded human lung adenocarcinoma tissue.