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    當前位置 : Millipore >>> Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells
    Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells
    • Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

    Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

    價格: ¥6288.00 市場價: 10480.00

    貨號: NS830
    品牌: Millipore
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      • Description
        CatalogueNumberNS830
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionGFAPELISAKit
        OverviewBackground
        GlialFibrillaryAcidicProtein(GFAP)isaclass-IIIintermediatefilamentuniquelyandubiquitouslyexpressedaspartofthestructuralnetworkinastrocyticglialcells.ThisspecificexpressionmakesGFAPidealasabroadastrocyticMarkerinvertebrates.Astrocytesplayavarietyofkeyrolesinsupporting,guiding,nurturing,andsignalingneuronalarchitectureandactivityinahighlyinterdependentmanner.CurrentunderstandingofCNSstructuresuggeststhatthereareatleastasmanyglialcellsastheestimated100billionneurons,butperhapsupwardsof100timesmore.Thus,
        thehealthofbothneuronsandastrocytesisoftenaffectedindiseaseandtrauma.GFAPisprovingtobeausefulbiomarkerinthedetectionofneuraldegeneration.AlthoughdefectsinGFAPhavebeenreportedtocausecertainrareconditions,suchasAlexander’sdisease(Brenneretal.,2001),theobservedinterconnectedphysiologyofneuronalandglialcellshasenabledtheuseofGFAPasaneurodegenerationdetectiongauge.Thisrelationshipisbeingexploitedbyresearchersandcliniciansmeasuringavarietyofneurologicalissuesfromcancertotrauma.GFAPhasbeenusedasabiomarkerintheearlydetectionofchemicallyinducedcancer(Capoetal.,1997)andclassicalglioblastomas(Jungetal.,2007).ElevatedlevelsofGFAPhavebeenreportedintraumaticbraininjury(TBI)andappeartodirectlycorrelatewiththeseverityofinjuryandbraintissueoxygenationincombatcasualtycareresearch(CCR,2008)aswellascivilianaccidents(Mondelloetal.,2010).GFAPhasalsobeenidentifiedasagoodbiomarkerinstudiesoftoxininducedacutebraininjury(Liaoetal.,2008),indifferentiationofhemorrhagicvs.ischemicstrokes(Foerchetal.,2006),aswellasincardiacarrest(Kanekoetal.,2009).MilliporenowoffersanELISAassayforsensitivemeasurementofGFAPproteinintissuehomogenatesandBIOLOGicalfluids(serum,plasma,CSF,andserumfreemedia)fromhuman,mouse,andrat.

        PrincipalsofProcedure
        ThisassayisaSandwichELISAbasedsequentiallyon:1)captureofsolubleGFAPproteintothewellsofamicrotiterplatecoatedbyapre-titeredamountofanti-GFAPmonoclonalantibody;2)removalofunboundmaterialbywashing;3)bindingofbiotinylatedanti-GFAPmonoclonalantibodyto
        thepreviouslycapturedGFAPprotein;4)removalofunboundmaterialbywashing;5)bindingofstreptavidinconjugatedhorserADIshperoxidasetothebiotinylatedantibodies;6)removalofexcessorunboundenzymeconjugatesbywashing;and7)quantificationofimmobilizedantibody-enzyme
        complexesbymonitoringhorseradishperoxidaseactivityinthepresenceofthesubstrate3,3’,5,5’-tetramethylbenzidine(TMB).Enzymeactivityismeasuredspectrophotometricallybyrecordingabsorbancereadingsat450nm.Increasesinabsorbencyaredirectlyproportionaltotheamountof
        GFAPproteincapturedfromthesample.ThereforetheproteinconcentrationofunknownscanbederivedbyinterpolationusingastandardcurvegeneratedinthesameassaywithreferencestandardsofknownconcentrationsofGFAP.
        AlternateNames
        • GFAP
        • GlialFibrillaryAcidicProteinELISA
        MaterialsRequiredbutNotDelivered1.Absorbentpaperorcloth
        2.Conventional,multi-channel,orrepeatingPipettes
        3.Reagentreservoirs
        4.Orbitalmicrotiterplateshaker/washer(optional)
        5.GraduatedSEROlogicalpipettes
        6.Microtiterplatereadercapableofreadingabsorbencyat450nm
        7.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
        8.Microfugetubesforstandardandsampledilutions
        9.Mechanicalvortex
        10.Milli-Q?Water
        ProductInformation
        Components
        • 96-WellAnti-GFAPCoatedPlate(CS206352):1Plate
        • 1XAssayBuffer(CS206357):40mL
        • 10XWashBuffer(CS206360):50mL
        • GFAPProteinStandard(CS206361):1Vial
        • GFAPQualityControl#1(CS206351):1Vial
        • GFAPQualityControl#2(CS206475):1Vial
        • BiotinylatedAnti-GFAPDetectionAntibody(CS206356):12mL
        • EnzymeSolution(CS206358):12mL
        • TMBSolution(CS202682):10mL
        • StopSolution(60193):12mL
        • PlateSealers(CS200443):10Sheets
        DetectionmethodChromogenic
        StorageandShippingInformation
        StorageConditionsStorage:TheGFAPELISAkitisshippedoniceandshouldbestoredat2-8°Cuponarrival.
        StABIlity:Kitcomponentsarestableforaminimumof6monthsfromthedateofreceiptifstoredand
        handledasdescribedabove.
        Applications
        KeyApplications
        • ELISA
        BiologicalInformation
        SpeciesReactivity
        • Mouse
        • Rat
        • Human
        AnalytesAvailable
        • GFAP
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Alternativesplicingresultsinmultipletranscriptvariantsencodingdistinctisoforms.
        GeneSymbol
        • GFAP
        UniProtNumber
        UniProtSummaryFUNCTION:GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells.
        SUBCELLULARLOCATION:Cytoplasm.Note:Associatedwithintermediatefilaments.
        TISSUESPECIFICITY:Expressedincellslackingfibronectin.
        PTM:PhosphorylatedbyPKN1.
        SIMILARITY:Belongstotheintermediatefilamentfamily.
        PhysicochemicalInformation
        Sensitivity
        • 1.5ng/mL
        StandardCurveRange
        • 1.5to100ng/mL
        Dimensions
        MaterialsInformation
        MaterialsInformation
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