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    當前位置 : Millipore >>> Millipore/NS220 | Neurite Outgrowth Assay Kit (3 μm)/NS220/12 assays
    Millipore/NS220 | Neurite Outgrowth Assay Kit (3 μm)/NS220/12 assays
    • Millipore/NS220 | Neurite Outgrowth Assay Kit (3 μm)/NS220/12 assays

    Millipore/NS220 | Neurite Outgrowth Assay Kit (3 μm)/NS220/12 assays

    價格: ¥3564.00 市場價: 5940.00

    貨號: NS220
    品牌: Millipore
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      • Description
        CatalogueNumberNS220
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionNeuriteOutgrowthAssayKit(3μm)
        OverviewApplication

        CHEMICON"sNS220NeuriteOutgrowthAssayKit(3μm)utilizesmicroporoustissuecultureinserttechnologyfromMilliporeandisbasedontheuseofMillicellcellcultureinserts(chambers)containingapermeablemembranewith3-μmporesatthebase.TheMillicellinsertsutilizedinthiskitareappropriateforcellsprojectingneuritesofupto3μmdiameter,e.g.N1E-115cells[Abeetal.,2003],DorsalRootGangliaandSchwanncells[Windebanketal.,1985].Theinsertsaredesignedtofitintoareceivervessel,whichwheninusecontainsdifferentiationmediaincontactwiththebottomoftheinsertmembrane.Thepermeablemembranesallowfordiscriminationbetweenneuritesandcellbodies,asprojectingneuriteswillpasseasilythroughtheporesbutcellbodieswillnot.Therefore,byinducingneuritestotraversethesemembranepores,apurifiedpopulationofneuritesislocatedontheundersideofthemembrane,distaltothesurfaceonwhichneuralcellbodiesaredeposited.
        Theassayisasimple,efficient,andversatilesystemforthequantitativedeterminationofcompoundsthatinfluenceneuriteformationandrepulsion.Withthissystem,itispossIBLetoscreennumerousBIOLOGicalandpharmacologicalagentssimultaneously,directlyevaluateadhesionandguidancereceptorfunctionsresponsibleforneuriteextensionandrepulsion,aswellasanalyzegenefunctionintransfectedcells.Themicroporousfilterallowsforbiochemicalseparationandpurificationofneuritesandcellbodiesfordetailedmolecularanalysisofproteinexpression,signaltransductionprocessesandidentificationofdrugtargetsthatregulateneuriteoutgrowthorretractionprocesses.ReagentsandmaterialssuppliedintheNS220NeuriteOutgrowthAssayKitaresufficientfor12tests.
        Note:the3μmporesizeinsertsinthiskitareunsuitableforusewithPC12cells.DuetotherelativelysmalldiameterofPC12cellbodies[Abeetal.,2003],someofthesecellsmaypassthough3μmporesandobscuretheresults.Chemicon"sNS225NeuriteOutgrowthAssayKit(1μm)hasbeenspecificallydesignedforandvalidatedwithPC12cells.
        MaterialsRequiredbutNotDelivered1.Desiredextracellularmatrixmembranecoatingprotein,e.g.,bovinecollagenIormouselaminin.
        2.Tissuecultureinstruments/supplies(including37°Cincubator,growthmedia,flasks/plates,detachmentbuffer,etc.)forcelltypeofinterest.
        3.Differentiationmedia/factorsforcelltypeofinterest(e.g.,nervegrowthfactor,serum-freemedia).
        4.1Xphosphatebufferedsaline(PBS)withCa2+/Mg2+.
        5.Invertedmicroscopeforneuritevisualizationonmembraneinserts.
        6.Parafilmandspectrophotometerforneuritestainextractionandquantification.
        7.Proteinlysisbuffer(optional)
        ProductInformation
        Components
        • 1.NeuriteOutgrowthPlateAssembly,3μm:(PartNo.2007256)1x24-wellplatecontaining12xMillicellhanginginsertswith3μmporesizemembranes.
        • 2.NeuriteStainSolution:(PartNo.90242)One20mLbottle.
        • 3.NeuriteStainExtractionBuffer:(PartNo.90243)One20mLbottle.
        • 4.NeuriteOutgrowthAssayPlate:(PartNo.2007255)Two24-wellplates.
        • 5.CottonSwabs:(PartNo.10202)50swabs.
        • 6.Forceps:(PartNo.10203)Oneeach.
        DetectionmethodChromogenic
        StorageandShippingInformation
        StorageConditionsWhenstoredat2-8°C,thekitcomponentsarestableuptotheexpirationdate.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.
        Applications
        ApplicationTheNS220NeuriteOutgrowthAssayKit(3μm)isbasedontheuseofMillicellcellcultureinserts(chambers)containingapermeablemembranewith3-μmporesatthebase.
        KeyApplications
        • Neuroscience
        BiologicalInformation
        SpeciesReactivity
        • All
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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