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A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.NEB also offers a Quick-Load version of this ladder with purple dye.
Recommended gel percentage range: 1.2-3%
Optimum separation on 2%
100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 μg/lane.
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications: