8-sulfhydrylguanosine and 8-OHG demonstrate some cross-reactivity at high concentrations (at least 2 orders of magnitude were required for positive cross-reactivity).
Form:
Lyophilized
Isotype:
IgG1
Recommend Usage:
Immunohistochemistry (1-10 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 50 mM TBS (1% BSA)
Storage Instruction:
Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Publication Reference
1.
SUMO2 regulates vascular endothelial function and oxidative stress in mice.Kim YR, Jacobs JS, Li Q, Gaddam RR, Vikram A, Liu J, Kassan M, Irani K, Kumar S.Am J Physiol Heart Circ Physiol. 2019 Dec 1;317(6):H1292-H1300. doi: 10.1152/ajpheart.00530.2019. Epub 2019 Oct 4.
2.
Protection against pyrimidine dimers, p53, and 8-hydroxy-2"-deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens.Liardet S, Scaletta C, Panizzon R, Hohlfeld P, Laurent-Applegate L.J Invest Dermatol. 2001 Dec;117(6):1437-41.
3.
Formation of 8-hydroxy-2"-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products.Yamagami K, Yamamoto Y, Kume M, Ishikawa Y, Yamaoka Y, Hiai H, Toyokuni S.Antioxid Redox Signal. 2000 Spring;2(1):127-36.
4.
Age and organ dependent spontaneous generation of nuclear 8-hydroxydeoxyguanosine in male Fischer 344 rats.Nakae D, Akai H, Kishida H, Kusuoka O, Tsutsumi M, Konishi Y.Lab Invest. 2000 Feb;80(2):249-61.
5.
Intranuclear distribution of 8-hydroxy-2"-deoxyguanosine. An immunocytochemical study.Toyokuni S, Iwasa Y, Kondo S, Tanaka T, Ochi H, Hiai H.J Histochem Cytochem. 1999 Jun;47(6):833-6.
6.
Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats, a model of type 2 diabetes.Ihara Y, Toyokuni S, Uchida K, Odaka H, Tanaka T, Ikeda H, Hiai H, Seino Y, Yamada Y.Diabetes. 1999 Apr;48(4):927-32.
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