| Immunogen | CelllineexpressingatruncatedformofNG2 | 
| Clone | 132.39 | 
| Host | Mouse | 
| Specificity | Thisantibodyrecognizesthenative,recombinant,andproteinfromcellswhichexpressNG2. | 
| Isotype | IgG1 | 
| SpeciesReactivity |  | 
| SpeciesReactivityNote | DemonstratedtoreactwithRat.ExpectedtoreactwithMouseandHumanbasedonsequencesimilarty. | 
| AntibodyType | MonoclonalAntibody | 
| EntrezGeneNumber |  | 
| EntrezGeneSummary | Extracellularmatrixproteinsecretedbymanycelltypes;memberofthelargechondroitinsulfateproteoglycan(CSPG)family;maybeinvolvedwithangiogenesis-dependentprocessessuchaswoundhealingortumorgrowth[RGD]. | 
| GeneSymbol |  | 
| PurificationMethod | ProteinGPurified | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsABIlitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreADIngbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades.
  SIZE:2326aminoacids;251909Da
  SUBUNIT:InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16(Bysimilarity).InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.
  SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein;Extracellularside.Apicalcellmembrane;Single-passtypeImembraneprotein;Extracellularside.Cellprojection?lamellipodiummembrane;Single-passtypeImembraneprotein;Extracellularside.Note=Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity).LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Afractionmayundergocellsurfaceproteolysisandsecretion.
  TISSUESPECIFICITY:Neuralcellsandalsoextraneuraltissues,especiallyinthedevelopingmesenchyme.
  PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation.InvolvedininteractionwithMMP16andITGA4.PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility.
  MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells. | 
| MolecularWeight | Theunconjugatedparentantibody(Cat.No.MAB5384)hasanobservedmolecularweightat~280kDa. |