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    當前位置 : Millipore >>> Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL
    Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL
    • Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

    Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

    價格: ¥4308.00 市場價: 7180.00

    貨號: MAB5304
    品牌: Millipore
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      • Description
        CatalogueNumberMAB5304
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-GlutamateAntibody
        ProductInformation
        FormatPurified
        PresentationLiquidinPBScontaining10mMsodiumazide.
        StorageandShippingInformation
        StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6monthsafterdate.
        Applications
        ApplicationAnti-GlutamateAntibodydetectslevelofGlutamate&hasbeenpublished&validatedforuseinIH.
        KeyApplications
        • Immunohistochemistry
        ApplicationNotesImmunohistochemistry:1:2,500-1:10,000usingfreefloatingsectionsbythePAPtechniqueonrathippocampus.

        Optimalworkingdilutionsmustbedeterminedbyenduser.

        PROTOCOLforGlutamateDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain.

        1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded.

        SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2.

        SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5.

        2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min.

        3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC).

        4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused.

        5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride.

        6.APPLICATIONOFGLUTAMATEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+2-8°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure.

        7.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-mouseinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor1-2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionC.

        8.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionCfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionC.

        9.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia.

        Forresearchuseonly;notforuseasadiagnostic.
        BIOLOGicalInformation
        ImmunogenGlutamate-Glutaraldehyde-BSA.
        HostMouse
        SpecificityGlutamate

        Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds:

        CompoundCross-reactivity

        Glutamate-G-BSA1

        Aspartate-G-BSA1/100,000

        GABA-G-BSA1/100,000

        Glutamate1/100,000

        Abbreviations:

        (G)Glutaraldehyde

        (BSA)BovineSerumAlbumin

        NOTE:AntibodyreactivityREQUIRESglutaraldehydefixationthussomeglutaraldehyde(0.5%-2.0%)needstobeincludedinthetissuefixationprocedureinorderfortheproperreactivity.
        IsotypeIgM
        SpeciesReactivity
        • Rat
        AntibodyTypeMonoclonalAntibody
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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