| Immunogen | Recombinantfull-lengthhumancaspase-3protein |
| Epitope | largesubunit&proform |
| Clone | 4-1-18 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Recognizesthefulllength32kDacaspase-3protein.Highlyspecifictocaspase-3andshowsnocross-reactionwithothercaspasefamilymembers.Caspase-3isubiquitouslyexpressedandlikeothercaspasesissynthesizedasaninactiveproenzyme.Uponactivation,caspase-3iscleavedgeneratingtwosmallersubunitsof17kDaand12kDa.Caspase-3isoneofthemostextensivelystudiedenzymeinapoptosis.Activationofcaspase-3occursinresponsetoavarietyofapoptoticinducers. |
| Isotype | IgG2aκ |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein. |
| GeneSymbol | - CASP3
- apopain
- CASP-3
- procaspase3
- CPP32B
- SCA-1
- CPP-32
- Apopain
- CPP32
- Yama
- EC3.4.22.56[Contains:Caspase-3subunitp17
- Caspase-3subunitp12].
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin. SIZE:277aminoacids;31608Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem. PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa. SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family. |