| Immunogen | TheantibodyepitopewasmappedtoasitewithinaconsensustyrosinesulfationmotifofPSGL-1,previouslyshowntobeessentialforinteractionwithP-selectin(andnowshowntobeessentialforrecognitionofPSGL-1byL-selectin). |
| Clone | KPL-1 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | P-selectinglycoproteinligand-1(PSGL-1).TheantibodyepitopewasmappedtoasitewithinaconsensustyrosinesulfationmotifofPSGL-1,previouslyshowntobeessentialforinteractionwithP-selectin(andnowshowntobeessentialforrecognitionofPSGL-1byL-selectin).{SnappKRetal.1998}.ThebindingofKPL-1tohumanPSGL-1onHL-60cellsdoesnotrequiresulfation{Thatte,Aetal2002}. |
| Isotype | IgG1κ |
| SpeciesReactivity | |
| SpeciesReactivityNote | KnownnottoreactwithRat,Bovine,Pig,andEquinePSGL-1{Snapp,KRetal1998;Baisse,Betal2007}. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | SELPLGisthehighaffinitycounter-receptorforP-selectinonmyeloidcellsandstimulatedTlymphocytes.Assuch,itplaysacriticalroleinthetetheringofthesecellstoactivatedplateletsorendotheliaexpressingP-selectin.TheorganizationoftheSELPGgenecloselyresemblesthatofCD43andthehumanplateletglycoproteinGpIb-alphabothofwhichhaveanintroninthe5-prime-noncodingregion,alongsecondexoncontainingthecompletecodingregion,andTATA-lesspromoters. |
| GeneSymbol | - SELPLG
- CLA
- PSGL-1
- CD162
- PSGL1
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q14242#BindstoP-,E-andL-selectins.Thecalcium-dependenthighaffinityinteractionwithP-selectinmediatesthetetheringandrollingofneutrophilsandT-lymphocytesonendothelialcells. SIZE:412aminoacids;43201Da SUBUNIT:Homodimer;disulfide-linked. SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Expressedonneutrophils,monocytesandmostlymphocytes. PTM:Displayscomplex,sialylatedandfucosylatedO-linkedoligosaccharides,atleastsomeofwhichappeartocontainpoly-N-acetyllactosaminewithvaryingdegreesofsubstitution.AlsoN-glycosylated.&SulfatedintheN-terminalregion;sulfationisnecessaryforP-selectinbinding. |