| ApplicationNotes | Immunohistochemistryondeparaffinizedsectionsofkidney,duodenum,andlivertissue:1:1000-1:1250.
  Flowcytometryon100μLof106cells:1:5000.
  Optimaldilutionsmustbedeterminedbytheenduser.
  IMMUNOHISTOCHEMICALPROCEDURE
  FORPARAFFINEMBEDDEDTISSUESECTIONS
  STAININGPROCEDURE:
  1.Deparaffinizesectionsinxylene3x5minutes.ResinembeddedorGMA(Glycolmethacrylate,2-hydroxyethylmethacrylate)treatedsectionsitisunnecessarytodeparaffinizeasthereisnoparaffin.
  2.Placeslidesingradedalcohols(100,95,90%)towaterat2minuteintervals,andairdry.(OmitforGMAsections)
  3.CirclesectionswithPAPpenordiamondpentoidentifyanddrythoroughly.
  4.ForGMAorresinsections,placeslidesin0.5%Tween/PBS(pH7.6)for29minutes.
  5.Incubateinprewarmed(40°C)1NHClfor1hour.
  6.Wash3x3minuteswithPBS(pH7.6).Slidesmaybeheldatthispointandtheprocedurecontinuedthefollowingday.
  7.Incubatein1XTrypsinsolution(0.02-0.05%w/v,prewarmedto40°C)for20minutesatroomtemperatureforNBF(normalbufferedformalin)fixedtissueor10minutesforCarnoy"sfixedtissue.
  8.Wash3x5minuteswithPBS(pH7.6).
  9.Incubatesectionsin1%H2O2(10mL30%H2O2in290mLmethanol)for20minutes,orGMAsectionsin3%H2O2(10mL30%H2O2in90mLwater)for3minutes.
  10.Wash2x2minuteswithPBS(pH7.6).
  11.UsingtheVectorABCkit,add3dropsofnormalhorseserumto10mLofPBS.Usethissolutiontoblocktheslidesfor20minutesatroomtemperature.
  12.BlottheslidesofexcessnormalhorseserumandincubatewithMAB4072dilutedinPBS/BSA/Tween20for1houratroomtemperature.
  13.Wash3x3minuteswithPBS(pH7.6).
  14.Add3dropsofnormalhorseserumto10mLofPBSandthenadd1dropofbiotinylatedantibodystock.Incubatetheslideswiththisdilutedsecondantibodyfor30minutesatroomtemperature.
  15.PreparetheVectastainABCReagentbyadding2dropsofreagentAto5mLofPBS.Thenadd2dropsofReagentBtothesamemixingbottle.Allowtositforabout30minutespriortouse.
  16.Wash3x3minuteswithPBS(pH7.6).
  17.IncubatetheslideswithABCReagentfor30minutesatroomtemperature.
  18.Wash3x3minuteswithPBS(pH7.6).
  19.Preparethesubstratebymixinganequalvolumeof0.02%H2O2(madeindistilledwaterfroma30%stocksolution)and0.1%DABmadein0.1MTrisbuffer,pH7.2.TheH2O2shouldbefreshlypreparedfromconcentratedstock.BecausemanyperoxidesubstratesareunstableinthepresenceofH2O2orwhenexposedtolight,thesubstrateshouldbepreparedjustpriortouse.SinceDABisasUSPectedcarcinogen,careshouldbetakeninhandlinganddisposingofallperoxidasesubstrates.
  20.StaintheslideswithDABsubstratefor2-5minutes.
  21.Wash2x2minuteswithdistilledwater.
  22.Counterstainwithhemotoxylin,dehydratethroughxylene,andmountthecoverslip. |