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Millipore/MAB377 | Anti-NeuN Antibody, clone A60/MAB377/500 µg

價格: ￥4440.00 市場價: 7400.00

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貨號: MAB377

品牌: Millipore

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Description
CatalogueNumber	MAB377
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-NeuNAntibody,cloneA60
AlternateNames	Neuron-SpecificNuclearProtein Neuna60 A60
BackgroundInformation	NeuNantibody(NEUronalNuclei;cloneA60)specificallyrecognizestheDNA-binding,neuron-specificproteinNeuN,whichispresentinmostCNSandPNSneuronalcelltypesofallvertebratestested.NeuNproteindistributionsareapparentlyrestrictedtoneuronalnuclei,perikaryaandsomeproximalneuronalprocessesinbothfetalandadultbrainalthough,someneuronsfailtoberecognizedbyNeuNatallages:INLretinalcells,Cajal-Retziuscells,Purkinjecells,inferiorolivaryanddentatenucleusneurons,andsympatheticganglioncellsareexamples(Mullenetal.,1992;Wolfetal.,1996).ImmunohistochemicallydetectableNeuNproteinfirstappearsatdevelopmentaltimepointsthatcorrespondwiththewithdrawaloftheneuronfromthecellcycleand/orwiththeinitiationofterminaldifferentiationoftheneuron(Mullenetal.,1992).ImmunoreactivityappearsaroundE9.5inthemouseneuraltubeandisextensivethroughoutthedevelopingnervoussystembyE12.5.Strongnuclearstainingsuggestsanuclearregulatoryproteinfunction;however,noevidencecurrentlyexistsastowhethertheNeuNproteinantigenhasafunctioninthedistalcytoplasmorwhetheritismerelysynthesizedtherebeforebeingtransportedbackintothenucleus.Nodifferencebetweenproteinisolatedfrompurifiednucleiandwholebrainextractonimmunoblotshasbeenfound(Mullenetal.,1992).

ProductInformation
Format	Purified
Control	Positivecontrol-BrainTissue.Negativecontrol-AnynonneuronaltissueegFibroblasts
Presentation	PurifiedmouseimmunoglobulinIgG1liquidinbuffercontaining0.02Mphosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor6monthsat2-8oCfromdateofreceipt.

Applications
Application	Anti-NeuNAntibody,cloneA60detectslevelofNeuNandhasbeenpublishedandvalidatedforuseinFC,IC,IF,IH,IH(P),IPandWB.
KeyApplications	FlowCytometry Immunocytochemistry Immunofluorescence Immunohistochemistry Immunohistochemistry(Paraffin) Immunoprecipitation WesternBlotting
ApplicationNotes	WesternBlotAnalysis: Apreviouslotofthisantibodyrecognized2-3bandsinthe46-48kDarangeandpossIBLyanotherbandatapproximately66kDa. Immunocytochemistry: 1:10-1:100dilutionfromapreviouslotwasused.Neuronsincultureshouldbepermeablizedwith0.1%tritonX-100.Allprimaryantibodydilutionsshouldbeperformedwithsimplesolutionscontainingonlybufferandprimaryantibodywithoutexcessproteinblocksordetergents. Immunohistochemistry: 1:100-1:1,000.Theantibodyworksbestonpolyesterwaxembeddedtissuebutalsoworksonparaffinembeddedtissueatalowerworkingdilution.Theantibodyworkswellwithformaldehyde-basedfixatives.Citricacidandmicrowavepretreatmenthasbeenusedsuccessfully(Sarnat,1998). Immunohistochemistry(paraffin)Analysis:ApreviouslotwasusedforIH(P). Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	Purifiedcellnucleifrommousebrain
Clone	A60
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	Millipore"sexclusivemonoclonalantibodytovertebrateneuron-specificnuclearproteincalledNeuN(orNeuronalNuclei)reactswithmostneuronalcelltypesthroughoutthenervoussystemofmiceincludingcerebellum,cerebralcortex,hippocampus,thalamus,spinalcordandneuronsintheperipheralnervoussystemincludingdorsalrootganglia,sympatheticchaingangliaandentericganglia.Developmentally,immunoreactivityisfirstobservedshortlyafterneuronshavebecomepostmitotic,nostaininghasbeenobservedinproliferativezones.Theimmunohistochemicalstainingisprimarilylocalizedinthenucleusoftheneuronswithlighterstaininginthecytoplasm.ThefewcelltypesnotreactivewithMAB377includePurkinje,mitralandphotoreceptorcells.TheantibodyisanexcellentMarkerforneuronsinprimaryculturesandinretinoicacid-stimulatedP19cells.Itisalsousefulforidentifyingneuronsintransplants.
Isotype	IgG1
SpeciesReactivity	Avian Chicken Ferret Human Mouse Pig Rat Salamander
AntibodyType	MonoclonalAntibody
GeneSymbol	NeuN A58
PurificationMethod	ProteinAPurfied
MolecularWeight	46/48kDa

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Millipore/MAB377 | Anti-NeuN Antibody, clone A60/MAB377/500 µg

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