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Millipore/MAB345 | Anti-O4 Antibody, clone 81/MAB345/50 µg

價格: ￥4440.00 市場價: 7400.00

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貨號: MAB345

品牌: Millipore

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Description
CatalogueNumber	MAB345
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-O4Antibody,clone81
AlternateNames	Sulfatide
BackgroundInformation	Oligodendrocytesandastrocytesarederivedfromcommonprecursorcells,glioblasts(Sommer,1981).O-Antigensaresulfatides,whichfunctionasdifferentiationMarkersonthesurfaceofoligodendrocytesofthecentralnervoussystem.O4isformedpostnatally(Schachner,1981)andisamarkerforcellbodiesandprocessesofoligodendrocytestypesIandII(hairyeyeballtype).Duringoligodendrocytedifferentiation,O4occursinpro-oligodendrocytes,butnotinO-2A-Progenitorcells.O4occursfromday3onwardsincellculturesofembryonicmousebrain.Anti-O4canbeusedinmyelination,demyelinationandremyelinationstudiesandinregenerationexperiments.

ProductInformation
Format	Purified
Control	Ratcorticalstemcellsorday3cellculturesofbrainsfrommouseembryos
Presentation	Purifiedimmunoglobulinin0.05MPotassiumphosphatebuffer,pH8.0with0.3MNaCland0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Maintainfor1yearat2–8°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

Applications
Application	Anti-O4Antibody,clone81isanantibodyagainstO4foruseinIC,IHwithmorethan50productcitations.
KeyApplications	Immunocytochemistry Immunohistochemistry
ApplicationsNotRecommended	Immunoprecipitation WesternBlotting
ApplicationNotes	Immunohistochemistry:10-20μg/mLonunfixed,shockfrozentissue. Immunocytochemistry:10-20μg/mLoncellsfixedwith4%paraformaldehyde. Note:O4isasulfatide,whichcanbedissolvedoutofthemembranebyorganicsolvents;acetoneandmethanolshouldnotbeusedforfixation. Optimalworkingdilutionsmustbedeterminedbytheenduser. Immunohistochemistryprotocol 1.Preparesectionsfromunfixed,shockfrozentissue.Thesectionsshouldbe4-5μmthick.Placethesectionsonmicroscopeslides. 2.Washtheslidethreetimesfor5min.eachinPBSatroomtemperature. 3.Blockthenon-specificbindingsitesbyincubatingthesectionsinahumidchamberwith5%FCSatroomtemperaturefor30minutes. 4.Washtheslidesasdescribedinstep2. 5.CoverthesectionswithasufficientamountofMAB345(10-20μg/mLinPBS)andincubateinahumidchamberat37°Cforonehour. 6.WashtheslidesbrieflythreetimeswithPBS.Carefullydryaroundtheareatobestained. 7.Coverthesectionswithasufficientamountofanti-mouseIgM-fluoresceinsolutionandincubateinahumidchamberat37°Cforonehour. 8.Washtheslidesasdescribedinstep6. 9.Coverthesectionswithasuitableembeddingmedium,coverwithacoverslip,andexaminebyfluorescencemicroscopy. HRPorABCcanalsobeused. Optimalresultscanbeobtainedbytitratingtheprimaryandsecondaryantibodies Immunocytochemistry 1.Fixthepreparationswith4%paraformaldehyde(inPBS)atroomtemperaturefor10minutes.O4isasulfatidewhichcanbedissolvedoutofthemembranebyorganicsolvents;acetoneandmethanolshouldnotbeusedforfixation. 2.Washtheslidethreetimesfor5min.eachinPBSatroomtemperature. 3.Blockthenon-specificbindingsitesbyincubatingthesectionsinahumanchamberwith5%FCSatroomtemperaturefor30minutes. 4.Washtheslidesasdescribedinstep2. 5.CoverthesectionswithasufficientamountofMAB345(10-20μg/mLinPBS)andincubateinahumidchamberat37°Cforonehour. 6.WashtheslidesbrieflythreetimeswithPBS.Carefullydryaroundtheareatobestained. 7.Coverthesectionswithasufficientamountofanti-mouseIgM-fluoresceinsolutionandincubateinahumidchamberat37°Cforonehour. 8.Washtheslidesasdescribedinstep6. 9.Coverthesectionswithasuitableembeddingmedium,coverwithacoverslip,andexaminebyfluorescencemicroscopy. Note:Donotallowthepreparationstodryoutduringstaining.

BIOLOGicalInformation
Immunogen	Homogenateofwhitematterofcorpuscallosumfrombovinebrain.
Clone	81(alsoreferredtointheliteratureasmABO4)
Concentration	Varies,seelotspecificCoA
Host	Mouse
Specificity	RecognizesOligodendrocytemarkerO4.Alsoreactswithcertaingalactolipidsinsperm(seeAdditionalInformationlibraryforlist).
Isotype	IgM
SpeciesReactivity	Chicken Human Mouse Rat
AntibodyType	MonoclonalAntibody
PurificationMethod	AmmoniumSulfatePrecipitation

PhysicochemicalInformation

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MaterialsInformation

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Millipore/MAB345 | Anti-O4 Antibody, clone 81/MAB345/50 µg

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