| Immunogen | Bovinebrainmicrotubuleprotein. |
| Clone | AP20 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | RecognizesMAP-2(microtubuleassociatedprotein-2).MAP-2isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles(1).
MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2(2,3).MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds(4,5).
SDS-PAGEProfiles:InSDS-PAGEMAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately300kD.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier). |
| Isotype | IgG1 |
| SpeciesReactivity | - Bovine
- Chicken
- Human
- Mouse
- Rat
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstabilizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed. |
| GeneSymbol | - MAP2
- MAP2B
- MAP-2
- MAP2C
- DKFZp686I2148
- MAP2A
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules. SIZE:1827aminoacids;199539Da PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats. |