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    當前位置 : Millipore >>> Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg
    Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg
    • Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg

    Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg

    價格: ¥4812.00 市場價: 8020.00

    貨號: MAB3418
    品牌: Millipore
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      • Description
        CatalogueNumberMAB3418
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-MAP2Antibody,cloneAP20
        AlternateNames
        • MicrotubuleAssociatedProtein
        • MAP2Aand2B
        BackgroundInformationMAP-2(microtubuleassociatedprotein-2)isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainneuronmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles.MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2.MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds.(Caceres,1986;Dotti,1987).
        ProductInformation
        FormatPurified
        Control
        • Brain,Neuronalculture,HumanglioblastomaT98Gcells.
        PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.02MPhosphatebuffer,pH7.6,0.25MNaClwith0.1%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor6monthsat2-8°Cinundilutedaliquotsfromdateofreceipt.
        Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer’scap.
        Applications
        ApplicationAnti-MAP2Antibody,cloneAP20detectslevelofMAP2&hasbeenpublished&validatedforuseinIH&WB.
        KeyApplications
        • Immunohistochemistry
        • WesternBlotting
        ApplicationNotesWesternBlot:
        60ng/mL.InSDS-PAGE,MAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately280-300kDrepresentingtheMAP-2Aand-2Bisoformsbutnotthe70kDaMAP-2Cisoform.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier).Usersshouldrun4%-20%SDS-PAGEgrADIentgels.

        Immunohistochemistry:
        5μg/mL(4%PFAfixedfrozensections).

        Immunohistochemistry:
        5μg/mLofapreviouslotwasused.Anti-MAP-2canbeusedtostaintissue(brainorspinalcord)fixedwithparaformaldehyde.

        Optimalworkingconcentrationsmustbedeterminedbytheenduser.
        BIOLOGicalInformation
        ImmunogenBovinebrainmicrotubuleprotein.
        CloneAP20
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityMAB3418bindsspecificallytoMAP-2.
        IsotypeIgG
        SpeciesReactivity
        • Bovine
        • Chicken
        • Human
        • Mouse
        • Rat
        • Xenopus
        • Quail
        SpeciesReactivityNoteRatandmouse.Expectedtocross-reactwithhuman,cow,chicken,quail,andXenopus.
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstABIlizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed.
        GeneSymbol
        • MAP2
        • MAP2B
        • MAP-2
        • MAP2C
        • DKFZp686I2148
        • MAP2A
        PurificationMethodProteinAPurfied
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules.
        SIZE:1827aminoacids;199539Da
        PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR.
        SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats.
        MolecularWeight230kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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