| Immunogen | Glutaminesynthetasepurifiedfromsheepbrain. |
| Clone | GS-6 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | ReactsstronglywithasinglebandonWesternblotsofsheepbrainandratbrain.Glutaminesynthetase,anenzymethatcatalyzestheaminationofglutamicacidtoformglutamine,isfoundinmammalsasanoctamerofeightidentical45kDasubunits.GlutaminesynthetaseactivityhasbeenshowntobeausefulMarkerofastrocytesandanimportantdifferentiationfeatureinretina.Glutaminesynthetaseisalsoconsideredtobeakeyenzymeintherecyclingoftheneurotransmitterglutamate. |
| Isotype | IgG2a |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Glutamineisamainsourceofenergyandisinvolvedincellproliferation,inhibitionofapoptosis,andcellsignaling(Haberleetal.,2005[PubMed16267323]).Fetalglutaminerequirementsareveryhighanddependlargelyonactiveglutaminesynthesisandthereleaseofglutamineintothefetalcirculationbytheplacenta.Glutaminesynthetase(EC6.3.1.2),alsocalledglutamate-ammonialigase(GLUL),isexpressedthroughoutthebodyandplaysanimportantroleincontrollingbodypHandinremovingammoniafromthecirculation.TheenzymeclearsL-glutamate,themajorneurotransmitterinthecentralnervoussystem,fromneuronalsynapses(seereferencesinClancyetal.,1996[PubMed8975719]).[suppliedbyOMIM] |
| GeneSymbol | |
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P15104#CatalyzestheconversionofammoniaandL-glutamatetoL-glutamine. SIZE:373aminoacids;42064Da SUBUNIT:Homooctamer. SUBCELLULARLOCATION:Cytoplasm. DISEASE:SwissProt:P15104#DefectsinGLULarethecauseofcongenitalsystemicglutaminedeficiency(CSGD)[MIM:610015].CSGDisararedevelopmentaldisorderwithseverebrainmalformationresultinginmulti-organfailureandneonataldeath.Glutamineislargelyabsentfromaffectedpatientsserum,urineandcerebrospinalfluid. SIMILARITY:SwissProt:P15104##Belongstotheglutaminesynthetasefamily. |
| MolecularWeight | 45kDa |