• <menu id="uuusw"></menu>
  • <table id="uuusw"></table>
    當前位置 : Millipore >>> Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg
    Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg
    • Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg

    Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg

    價格: ¥3576.00 市場價: 5960.00

    貨號: MAB2081
    品牌: Millipore
    規格
    數量
    庫存(0)
    特別 提示
    代購產品:無質量問題不接受退換貨,下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格,訂單生效后再付款。
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616


    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題
      • Description
        CatalogueNumberMAB2081
        Replaces04-1002
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-β-CateninAntibody,clone5H10
        BackgroundInformationThetransmembrane,calciumdependentmoleculeE-cadherinisconsideredtobeoneofthekeymoleculesintheformationoftheintercellularjunctionalcomplexandestablishmentofpolarityinepithelialcells.Catenins,thealpha-subunit(102kDa),beta-subunit(88kDa)andgamma-subunit(82kDa),[reviewedinTakeichi,1991]areagroupofproteinsthatinteractwiththeintercellulardomainofE-cadherin,resultingincomplexesofE-cadherin/beta-catenin/alpha-cateninorE-cadherin/gamma-catenin/alpha-Catenin(Hincketal.,1994).Beta-catenin,thevertebratehomologueofDrosophilasegmentpolaritygenearmADIllo,andkeyelementoftheWntsignaltransductionpathways,isimportantinembryogenesis,celltransformation,andintercellularadhesionincludingtheadhesion,motilityandmetastasisofcancercells.Thus,cateninproteinexpression,regulationandlocalizationarepotentiallyimportantMarkerstopredictmotilityandinvasivenessofepithelialneoplasms.Theregionsofbothalpha-andbeta-catenin,locatedon5q21-22and3p21,havebeenshowntobeinvolvedinthedevelopmentofcertaintumors(seeJiang,1996;Schlosshaueretal.,2002)andreducedexpressionsofbothalpha-andbeta-cateninhavebeendescribedinvarioustumorsincludingbreastcarcinoma(Hashizumeetal,1996).Besidesadhesionfunctions,beta-cateninbindstothetumorsuppressorgeneAPC.APCmutationdisturbstheequilibriumandcytoplasmiclevelsoffreebeta-cateninlevelinthecellandmayhavearoleintumOrigenesis.
        ProductInformation
        FormatPurified
        Control
        • A431andHT1080celllineextracts
        PresentationProteinAPurifiedIgGinliquidat1mg/mLin0.02MPBwith0.25MNaCl,pH7.6,containing0.1%sodiumazide.
        StorageandShippingInformation
        StorageConditionsMaintainfor1yearat2–8°Cfromdateofshipment.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
        Applications
        ApplicationAnti-β-CateninAntibody,clone5H10isaMouseMonoclonalAntibodyfordetectionofβ-Cateninalsoknownasbetacatenin&hasbeentestedinICC,IHC,IHC(P),IP&WB.
        KeyApplications
        • Immunocytochemistry
        • Immunohistochemistry
        • Immunohistochemistry(Paraffin)
        • Immunoprecipitation
        • WesternBlotting
        ApplicationNotesImmunohistochemistry:Lowcross-linkingfixativesarerecommended.Histochoicefixativeiseffective.FormalinandPFAareeffective,howevermayshowweakerstaining.

        Immunohistochemistryonformalinfixedparaffinembeddedtissuesrequireslightfixationsandcitricacid/microwaveantigenrecoveryforstaining;Inbrief,sectionsof2μmthicknessweretreatedafterdeparaffinizationwith0.3%hydrogenperoxideinmethanolfor30minutestoinhibitendogenousperoxidaseandwashedwithTBSsolution(Tris-bufferedsalinepH7.6).Toenhanceantigenretrieval,sectionsweremicrowave-pretreatedin0.01Mcitratebuffersolutionat750Wfora3minute-cycleperiodrepeatedthreetimes;amplifieddetectionmethodsarerecommended.

        Immunocytochemistry:EffectiveforA431andHT1080celllineswithacetoneormethanol,orlightPFAfixation.

        Immunoblotting

        Immunoprecipitation

        Optimalworkingdilutionsmustbedeterminedbyend-user.
        BIOLOGicalInformation
        ImmunogenProducedbyimmunizationwithrecombinanthumanbeta-catenin.
        Clone5H10
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityRecognizesanepitopeinthecarboxyterminus,aminoacids758-771,ofhumanbeta-catenin.
        IsotypeIgG1
        SpeciesReactivity
        • Human
        • Rat
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryBeta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactinCytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM]
        GeneSymbol
        • CTNNB1
        • FLJ25606
        • CTNNB
        • Beta-catenin
        • PRO2286
        • FLJ37923
        • DKFZp686D02253
        PurificationMethodProteinAPurfied
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P35222#InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway.
        SIZE:781aminoacids;85497Da
        SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.ThestABIlizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2.InteractswithSLC30A9(Bysimilarity).
        SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2promotesnucleartranslocation.
        TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon.
        PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.&EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.&UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1X(Probable).Itsubiquitinationleadstoitssubsequentproteasomaldegradation.
        DISEASE:"SwissProt:P35222#ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.&DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.&DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.&AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1."
        SIMILARITY:SwissProt:P35222##Belongstothebeta-cateninfamily.&Contains12ARMrepeats.
        MolecularWeight94kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
      售后保障
      螞蟻淘生物188,秉承螞蟻淘一貫的嚴謹態度,由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后,保障正品優質。以“快速好省,為科研提供好產品、好價格”為理念,直接鏈接原廠家,從全國各地原制造商嚴格挑選188款科研精品,剔除品牌溢價,188生物新電商,把好的產品帶給科研!? 力求給你最優質的商品。
    • Q:生物188產品正品保障嗎?
      A:生物188質量把控人員具有十年的從業經驗,在業界享有良好的口碑;自營商城,直接從廠家采購, 自己的團隊負責國際物流和清關,中間沒有第三方,所有流程嚴格把控,100%保證正品,假一罰十。

      Q:下單后可以修改訂單嗎?
      A:下單后的商品付款之前可以修改;訂單付款成功,需要聯系我們客服進行修改;客服電話:4000-520-616

      Q:可以開發票嗎?
      A:本網站所售商品都是正規清關,均開具16%正規發票,發票金額含配送費金額,另有說明的除外。

      Q:商品幾天可以發貨?
      A:生物188商品,全部現貨銷售,付款后即可發貨,一般一周內送達!

      Q:如何聯系商家?
      A:有任何問題夠可以電話咨詢我們,全國24小時免費服務熱線:4000-520-616 或聯系我們的在線客服QQ:1570468124

      Q:收到的商品少了/發錯了怎么辦?
      A:同個訂單購買多個商品可能會分為一個以上包裹發出,可能不會同時送達,建議查看訂單詳情是否是 部分發貨狀態;如未收到,可聯系在線客服或者致電4000-520-616。

      Q:退換貨/維修需要多長時間?
      A:一般情況下,退貨處理周期為客戶收到產品一個月內(以快遞公司顯示簽收時間為準),包裝規格、 數量、品種不符,外觀毀損、短缺或缺陷,請在收到貨24小時內申請退換貨;特殊商品以合同條款為準。

    何為188

    極簡而嚴謹,我們僅銷售188款生物醫學科研用品,款款都是爆款;因為少所以聚焦,聚焦甄選每一款產品,聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知
    • 品質甄選,正品保證

    • 自營電商,廠家直采

    • 極簡主義,188精品

  • <menu id="uuusw"></menu>
  • <table id="uuusw"></table>
    上床视频