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    當前位置 : Millipore >>> Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL
    Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL
    • Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL

    Millipore/MAB2030 | Anti-Chondroitin 4 Sulfate Antibody, clone BE-123/MAB2030/100 µL

    價格: ¥3204.00 市場價: 5340.00

    貨號: MAB2030
    品牌: Millipore
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      • Description
        CatalogueNumberMAB2030
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-Chondroitin4SulfateAntibody,cloneBE-123
        ProductInformation
        FormatAscites
        PresentationAscitesfluidcontainingnopreservatives.
        StorageandShippingInformation
        StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
        Applications
        ApplicationAnti-Chondroitin4SulfateAntibody,cloneBE-123detectslevelofChondroitin4Sulfate&hasbeenpublished&validatedforuseinELISA,RIA&WB.
        KeyApplications
        • ELISA
        • RADIoimmunoassay
        • WesternBlotting
        ApplicationNotesELISAat1:400-1:3,200.

        RIAat1:50-1:6,400.

        Immunohistochemistry:Thisantibodyonlyreactswithdigestedmaterial.FixedtissuemustbedigestedwithchondroitinaseABC(Sigma).
        TheReference23757342shouldbeindicatedforIHC.(Reine,TM.,etal.,PubMedID:23757342)

        WesternBlot:1:1000-1:4000usingECLdetectionmethod.ChondroitinaseABCtreatmentoflysatesampleishelpful.
        Inanyalkalibuffer,e.g.Trisoracetate(50mMor100mM)pH7.4-7.6.MakeastocksolutionbydilutingonevialofSigmaChondroitinaseA,B,C,(5units/vial)in100μL.Foruse,dilutestock10-foldintothebufferofchoice.Useataratioof50-100mgofproteoglycanper5unitsofenzyme.Onfixedcells,diluteinTrisacetateandonnon-fixedcellsydilute10-25foldinDMEM.
        Poole,C.A.,etal.,J.Histochem.Cytochem.1991.39:1175-1187.

        Optimalworkingdilutionsmustbedeterminedbytheenduser.
        BIOLOGicalInformation
        ImmunogenMouseproteoglycan.
        CloneBE-123
        HostMouse
        SpecificityReactswithbothmouse(fetalandadult)andhuman(fetalandadult)chondroitinaseABCdigestedcartilageproteoglycans.Chondroitin-4sulfatedisaccharidessuccessfullyinhibitthebindingofmonoclonalantibodytoproteoglycans.NoreactionwithnativeproteoglycansorwithproteoglycansdigestedwitheitherchondroitinaseACortesticularhyaluronidase.
        IsotypeIgG1κ
        SpeciesReactivity
        • Bovine
        • Canine
        • Human
        • Mouse
        • Rat
        • Sheep
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryAhumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreadingonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells.
        GeneSymbol
        • CSPG4
        • MSK16
        • MCSP
        • NG2
        • MCSPG
        • MEL-CSPG
        • HMW-MAA
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades.
        SIZE:2322aminoacids;250497Da
        SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16.
        SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity).
        TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells.
        PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity).
        SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains.
        MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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