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    當前位置 : Millipore >>> Millipore/MAB1407P | Anti-Macrophages/Granulocytes Antibody, clone OX-41/MAB1407P/100 µg
    Millipore/MAB1407P | Anti-Macrophages/Granulocytes Antibody, clone OX-41/MAB1407P/100 µg
    • Millipore/MAB1407P | Anti-Macrophages/Granulocytes Antibody, clone OX-41/MAB1407P/100 µg

    Millipore/MAB1407P | Anti-Macrophages/Granulocytes Antibody, clone OX-41/MAB1407P/100 µg

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    貨號: MAB1407P
    品牌: Millipore
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      • Description
        CatalogueNumberMAB1407P
        ReplacesMAB1407
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-Macrophages/GranulocytesAntibody,cloneOX-41
        AlternateNames
        • CD172antigen-likefamilymemberA
        • CD172aantigen
        • InhibitoryreceptorSHPS-1
        • Macrophagefusionreceptor
        • MyD-1antigen
        • SHPsubstrate1
        • SHPsubstrate-1
        • Signal-regulatoryproteinalpha-1
        • signal-regulatoryproteinalpha
        BackgroundInformationMacrophagesandgranulocytesexpressSignalregulatoryprotein(SIRP),whichisatransmembraneglycoproteininvolvedinthedownregulationoftyrosinekinasereceptorsignaltransductioninducedbycelladhesion,growthfactorsorinsulin.Theyareimmunoglobulin-likecellsurfacereceptorforCD47.ByCD47bindingthereisanegativeregulationofphagocytosis,mastcellactivationanddendriticcellactivationthatpreventsmaturationofimmaturedendriticcellsandinhibitscytokineproductionbymaturedendriticcells.
        ProductInformation
        FormatAffinityPurified
        Control
        • Macrophages,monocytes,lymphoidorgans.
        PresentationPurifiedmousemonoclonalIgG2ainbuffercontaining0.09%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor6monthsat2-8°Cinundilutedaliquotsfromdateofreceipt.
        Applications
        ApplicationThisAnti-Macrophages/GranulocytesAntibody,cloneOX-41isvalidatedforuseinFC,IC,IH,IH(P),WBforthedetectionofMacrophages/Granulocytes.
        KeyApplications
        • FlowCytometry
        • Immunocytochemistry
        • Immunohistochemistry
        • Immunohistochemistry(Paraffin)
        • WesternBlotting
        ApplicationNotesImmunohistochemistry:
        FrozensectionsandFormalinfixed,paraffinembeddedtissuesections(requirestrypsinorpronasepre-treatment).

        WesternBlotting:
        Apreviouslotofthisantibodywasusedinwesternblot.

        Flowcytometry:
        1:100-1:200ofapreviouslotwasused.(Use10μLofworkingdilutionfor10^6cellsin100μL).

        Optimalworkingdilutionsmustbedeterminedbytheenduser.
        BIOLOGicalInformation
        ImmunogenRatperitonealmacrophages.
        CloneOX-41
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityMAB1407PrecognizesratSIRP(Signalregulatoryprotein),whichisexpressedselectivelybymyeloidcellsandbyneurons.ThismonoclonalbindstoadifferentepitopeonSIRPthanED9.ItdoesnotblocktheinteractionofCD172aandCD47.
        Labelsmostmacrophagesincludingresidentperitoneal,alveolarandactivatedmacrophages.Recognitionofothermacrophagesislimitedtoonlyoccasionalkupffercells.Inadditionitlabelsgranulocytesandapopulationofdendriticcells.Italsolabelsbrainextensively.
        IsotypeIgG2a
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        SpeciesReactivityNoteHuman.Expectedtocross-reactwithratandmouse.
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryTheproteinencodedbythisgeneisamemberofthesignal-regulatory-protein(SIRP)family,andalsobelongstotheimmunoglobulinsuperfamily.SIRPfamilymembersarereceptor-typetransmembraneglycoproteinsknowntobeinvolvedinthenegativeregulationofreceptortyrosinekinase-coupledsignalingprocesses.Thisproteincanbephosphorylatedbytyrosinekinases.Thephospho-tyrosineresiduesofthisPTPhavebeenshowntorecruitSH2domaincontainingtyrosinephosphatases(PTP),andserveassubstratesofPTPs.Thisproteinwasfoundtoparticipateinsignaltransductionmediatedbyvariousgrowthfactorreceptors.CD47hasbeendemonstratedtobealigandforthisreceptorprotein.ThisgeneanditsproductshareveryhighsimilaritywithseveralothermembersoftheSIRPfamily.Theserelatedgenesarelocatedincloseproximitytoeachotheronchromosome20p13.Multiplealternativelysplicedtranscriptvariantshavebeendeterminedforthisgene.[providedbyRefSeq]
        GeneSymbol
        • CD172a
        • SIRPA
        • SIRPalpha2
        • BIT
        • SHPS1
        • MFR
        • SIRP
        • p84
        • SHPS-1
        • Sirp-alpha-1
        • CD172A
        • Sirp-alpha-2
        • SIRP-ALPHA-1
        • PTPNS1
        • SIRPalpha
        • P84
        • Bit
        • MYD1
        • MYD-1
        PurificationMethodImmunoAffinityPurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P78324#Immunoglobulin-likecellsurfacereceptorforCD47.ActsasdockingproteinandinducestranslocationofPTPN6,PTPN11andotherbindingpartnersfromthecytosoltotheplasmamembrane.Supportsadhesionofcerebellarneurons,neuriteoutgrowthandglialcellattachment.Mayplayakeyroleinintracellularsignalingduringsynaptogenesisandinsynapticfunction(Bysimilarity).Involvedinthenegativeregulationofreceptortyrosinekinase-coupledcellularresponsesinducedbycelladhesion,growthfactorsorinsulin.Mediatesnegativeregulationofphagocytosis,mastcellactivationanddendriticcellactivation.CD47bindingpreventsmaturationofimmaturedendriticcellsandinhibitscytokineproductionbymaturedendriticcells.
        SIZE:503aminoacids;54813Da
        SUBUNIT:BindsPTPN11whentyrosine-phosphorylated,exceptinmacrophages,whereitprimarilybindsPTPN6.BindsGRB2invitro.BindsFGR(Bysimilarity).BindsJAK2irrespectiveofitsphosphorylationstatusandformsastablecomplex.BindsSCAP1and/orSCAP2.TheresultingcomplexrecruitsFYB.BindsPTK2B.
        SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
        TISSUESPECIFICITY:Ubiquitous.Highlyexpressedinbrain.Detectedonmyeloidcells,butnotT-cells.Detectedatlowerlevelsinheart,placenta,lung,testis,ovary,colon,liver,smallintestine,prostate,spleen,kidney,skeletalmuscleandpancreas.
        PTM:N-glycosylated.&PhosphorylatedontyrosineresiduesinresponsetostimulationwithEGF,growthhormone,insulinandPDGF.DephosphorylatedbyPTPN11.
        SIMILARITY:SwissProt:P78324##Contains2Ig-likeC1-type(immunoglobulin-like)domains.&Contains1Ig-likeV-type(immunoglobulin-like)domain
        MolecularWeight56kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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