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T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. T4 DNA Ligase joins blunt end and cohesive end termini as well as repairs single stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
Immobilized T4 DNA Ligase (IM T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet, and can be re-used.
Temperature | % Activity |
|---|---|
16ºC | 100% |
25ºC | 100% |
37ºC | 50% |
Buffer | pH | % Activity |
|---|---|---|
NEBuffer r1.1 | 7.0 | 50% |
NEBuffer r 2.1 | 7.9 | 100% |
NEBuffer r3.1 | 7.9 | 70% |
rCutSmart Buffer | 7.9 | 100% |
Phi29 DNA Polymerase Buffer | 7.5 | 100% |
Standard Taq Buffer | 8.3 | 70% |
Thermopol Buffer | 8.8 | 70% |
Q5 Reaction Buffer | 8.5 | 10% |
T4 DNA LigaseBuffer | 7.5 | 100% |
NEBNext Quick Ligation Buffer | 7.6 | 25% |
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