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Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations (D355A, E357A) which abolish the 3´→ 5´ exonuclease activity (1).
Highlights
Isolated from a recombinant source
Generates probes using random primers
Dideoxy sequencing
Supplied with 10X Reaction Buffer
Moderate strand displacement activity
Product Source
AnE. colistrain containing a plasmid with a fragment of theE.colipolA (D355A, E357A) gene starting at codon 324.
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