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    當前位置 : Pierce >>> Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091
    Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091
    • Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091

    Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091

    價格: ¥5988.00 市場價: 9980.00

    貨號: KHO0091
    品牌: Pierce
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      • Description

        TheERK1/2[pTpY185/187]ELISAresearchuseonlykitisdesignedtodetectandquantifythelevelofERK2proteinphosphorylatedatthreonineresidue185andtyrosine187andERK1atthreonine202andtyrosine204.ThisassayisintendedforthedetectionofERK1/2[pTpY185/187]fromhumancelllysatesusing96-wellplatesandamicroplatereader.

        Easytorun
        Moresensitivethanwesterns
        Convenientprecoated,removable8-wellstrips

        Easy-to-RunSandwichELISA
        Thetotalassayincubationtimeisonly4hours.AmonoclonalcaptureantibodyspecificforERK1/2hasbeencoatedontothewellsofthe96-wellplateprovided.Duringthefirstincubation,standardsofknownERK1/2[pTpY185/187]contentandunknownsamplesarePipettedintothewellsandtheERK1/2antigenbindstotheimmobilized(capture)antibody.Afterwashing,arabbitantibodyspecificforERK1/2[pTpY185/187]isaddedtothewells.Duringthesecondincubation,thisantibodyservesasadetectionantibodybybindingtotheimmobilizedERK1/2proteincapturedduringthefirstincubation.Afterwashing,ahorserADIshperoxidase–labeledanti–rabbitIgGisadded.Thisbindstothedetectionantibodytocompletethefour–membersandwich.Afterathirdincubationandwashingtoremovealltheunboundenzyme,asubstratesolution(TMB)isadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofERK1/2[pTpY185/187]presentintheoriginalspecimenandtheopticaldensitycanbereadonastandardmicroplatereader.

        DetectLow-ExpressingProteinsandUseLessSample
        ELISAkitsdesignedtomeasureintracellularsignalingtargetsare2–10timesmoresensitivethanwesternblotting.Theimprovedsensitivityenablesyoutodetectlow-expressingproteinsthatotherwisemaynotbedistinguishablefrombackground.Inaddition,theamountofsampleneededtoruntheassayislessthanwhatisneededforwesternblots.

        Ready-to-UseERK1/2[pTpY185/187]ELISAKitContainsTheseReagents,Buffers,andSupplies:
        •ERK1/2AntibodyCoated96-WellPlate
        •ERK1/2[pTpY185/187]Standard
        •StandardDiluentBuffer
        •ERK1/2[pTpY185/187]DetectionAntibody
        •Anti-RabbitIgG-HRP(100X)
        •HRPDiluent
        •WashBufferConcentrate(25X)
        •StABIlizedChromogen,TMB
        •StopSolution
        •PlateCovers
        •Detailedprotocolwithvalidationtests

        ConvenientKitFormat
        Thekitcomeswithacaptureantibodyprecoatedtotheplate,eliminatinganovernightcoatingprocess.Theplatesaremachinecoatedtoprovidelowvariabilitywell-to-well.FurThermore,theplatecomesasremovable8-wellstripsallowingyoutorunasfewsamplesasyouwant.Inaddition,thestandardsprovidedinthekitcanbeusedtogetquantitativeresultsoffthestandardcurveand/orcanbeusedasapositivecontrol.

        RigorousValidation
        EachELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeprotocolinsertformoreinformationonvalidation.

        PartoftheELISAKitFamily
        WehaveawidemenuofELISAresearchuseonlykits.Withexcellentsensitivity,provenspecificity,andconsistentperformance,theseoptimizedELISAkitsofferyouachoicewhenitcomestogettingphysiologicallyrelevantandquantitativeresults.

        Forresearchuseonly.Notintendedforhumanoranimaltherapeuticordiagnosticuse.

        RelatedLinks:

        LearnmoreaboutELISAKits
        LearnmoreaboutotherImmunoassays
        ForResearchUseOnly.Notforuseindiagnosticprocedures.

        Figures

        Specifications

        ProteinFamily:Kinases&Inhibitors
        AssayIncubationTime:4hr
        AssayRange:1.6U/mL
        AssaySensitivity:<0.8Units/mL
        DetectionMethod:Colorimetric
        ForUseWith(Equipment):MicroplateReader
        FormerIdentifiers:Novex
        GeneAliases:ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK, ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK
        GeneID:5594, 5595
        GeneName:mitogen-activatedproteinkinase1, mitogen-activatedproteinkinase3
        GeneSymbol:MAPK1, MAPK3
        ImmunoassayKitFormat:CoatedELISAKit
        LabelorDye:HRP(HorseradishPeroxidase)
        ProductSize:96tests
        ProteinName:Mitogen-activatedproteinkinase1, Mitogen-activatedproteinkinase3
        ProteinSubtype:Serine⁄ThreonineKinases
        SampleType(Specific):CellExtracts
        SampleVolume:100µL
        ShippingCondition:WetIce
        Target(KitNamed):ERK1/ERK2
        TargetSite-Specificity:[pT185/pY187]
        TargetSpecies(Named):Human
        TargetSpecies(Validated):Human, Mouse, Rat
        TargetSub-Specificity:Phospho
        UniProtID:P27361, P28482

        Contents&storage

        Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.Seeproductmanualfordetailedcontentsandstorageconditionsformaximumstability.
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