• <menu id="uuusw"></menu>
  • <table id="uuusw"></table>
    當前位置 : Millipore >>> Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit
    Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit
    • Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit

    Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit

    價格: ¥6240.00 市場價: 10400.00

    貨號: FCCS025101
    品牌: Millipore
    規格
    數量
    庫存(0)
    特別 提示
    代購產品:無質量問題不接受退換貨,下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格,訂單生效后再付款。
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616


    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題
      • Description
        CatalogueNumberFCCS025101
        TradeName
        • FlowCellect
        DescriptionFlowCellect?EGFR/MAPKPathwayActivationDetectionKit
        OverviewMillipore’sFlowCellect?EGFR/MAPKActivationDetectionkitincludestwodirectlyconjugatedphosphospecificantibodies:pEGFR(Tyr1173)-AlexaFluor488andpERK(Thr202/Tyr204,Thr185/Tyr187)-PE,alongwithoptimizedfixation,permeABIlization,washandassaybufferstoprovideresearcherstheabilitytodetecttheactivationofEGFRandERKatthereceptoranddownstreamsignalinglevel.
        TheabilitytodetectthephosphorylationofEGFRandERKsimultaneouslyallowsresearcherstoprofileEGFmediatedMAPKactivationwithinacelltypeofinterest.Alterationsinthesesignalingprofilescanbeusedtomonitortheeffectofaparticularupstreamtreatmenttargetingthepathway.
        Antibodiesagainstphospho-EGFRandphospho-ERK1/2areprovidedinthekittomeasuretheextentofeachproteinsactivationstate.A431cellswereusedasamodelsystemtodemonstrateEGFmediatedactivationofEGFRaswellasits’downstreameffectorkinaseERK.Thetreatedcellswerecomparedtountreatedcellstoverifythedetectionofbothactivatedproteinsbyflowcytometry.Whenperformingadualcolorstain,themajorityofthetreatedcellsaredoublepositiveindicatingactivationofMAPKthroughEGFR.
        Sufficientreagentsareprovidedfor253-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.
        AlternateNames
        • FlowCellect
        BackgroundInformationTheEpidermalGrowthFactorReceptor(EGFR)isoneoftheReceptorTyrosineKinaseswhichcanactivatetheMAPKpathway,oneofthreemajorsignalingpathwaysdownstreamofEGFR.EGFRbelongstoErbBreceptorsuperfamily,agroupoftransmembraneglycoproteinreceptors,consistingoffourmembers:EGFR(ErbB-1),ErbB-2(c-neu,HER2),ErbB-3(HER3),andErbB-4(HER4).

        Criticalcellulardecisionssuchasproliferation,migration,anddifferentiation,areregulatedby
        stimulatorycuesfromtheextracellularenvironment,primarilygrowthfactors,extracellular
        matrix(ECM)proteins,andadhesionmoleculespresentedonthesurfaceofneighboringcells.Oncethesecuesarereceived,cellsundergocomplexsignalingcascadessuchastheMitogenActivatedProteinKinase(MAPK)pathwayinordertorespondtotheirenvironmentalstimuli.

        GrowthfactorsandmitogensusetheRas/Raf/MEK/ERKsignalingcascadetotransmitsignalsfrom
        theirreceptorstoregulategeneexpressionandpreventapoptosis.OnceactivatedbyphosphorylationExtra-cellularsignalRelatedKinase(ERK)entersthenucleusandactivatestargetproteinsassociatedwithcellularproliferationandmigration.

        DeregulationoftheEGFR/ERKpathwayduetoalterationsaffectingtheexpressionorfunctionofanumberofpathwaycomponentshaslongbeenassociatedwithnumerousformsofcancer.DuetothecomplexityofthepathwayanditsinvolvementinmanymalignanciestheEGFR/MAPKpathwayisstudiedbynumerousresearcherslookingforinsightintothemechanismsinvolvedintheformationandprogressionofcancers.
        ProductInformation
        Components
        • 1.20XAnti-phospho-EGFR(Tyr1173),clone9H2;AlexaFluor488Conjugate:(PartNo.CS204825)Onevialcontaining150mLantibody.
        • 2.20XAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187),cloneAW39R;R-PhycoerythrinConjugate:(PartNo.CS203329)Onevialcontaining150mLantibody.
        • 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mLbuffer.
        • 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mLbuffer.
        • 5.5XAssayBuffer:(PartNo.CS202124)Onebottlecontaining55mLbuffer.
        • 6.1XPermeabilizationBuffer:(PartNo.CS202125)Onebottlecontaining13mLbuffer.
        DetectionmethodFluorescent
        StorageandShippingInformation
        StorageConditions4-8°Cforantibodiesandbuffers
        Applications
        ApplicationThisFlowCellectEGFR/MAPKActivationDetectionKitforFlowCytometryprovidesresearcherstheabilitytodetecttheactivationofEGFR&ERKatthereceptor&downstreamsignalinglevel.
        KeyApplications
        • FlowCytometry
        BIOLOGicalInformation
        Clone9H2;AW39R
        HostMouse
        SpeciesReactivity
        • Human
        AntibodyTypeMonoclonalAntibody
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
      售后保障
      螞蟻淘生物188,秉承螞蟻淘一貫的嚴謹態度,由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后,保障正品優質。以“快速好省,為科研提供好產品、好價格”為理念,直接鏈接原廠家,從全國各地原制造商嚴格挑選188款科研精品,剔除品牌溢價,188生物新電商,把好的產品帶給科研!? 力求給你最優質的商品。
    • Q:生物188產品正品保障嗎?
      A:生物188質量把控人員具有十年的從業經驗,在業界享有良好的口碑;自營商城,直接從廠家采購, 自己的團隊負責國際物流和清關,中間沒有第三方,所有流程嚴格把控,100%保證正品,假一罰十。

      Q:下單后可以修改訂單嗎?
      A:下單后的商品付款之前可以修改;訂單付款成功,需要聯系我們客服進行修改;客服電話:4000-520-616

      Q:可以開發票嗎?
      A:本網站所售商品都是正規清關,均開具16%正規發票,發票金額含配送費金額,另有說明的除外。

      Q:商品幾天可以發貨?
      A:生物188商品,全部現貨銷售,付款后即可發貨,一般一周內送達!

      Q:如何聯系商家?
      A:有任何問題夠可以電話咨詢我們,全國24小時免費服務熱線:4000-520-616 或聯系我們的在線客服QQ:1570468124

      Q:收到的商品少了/發錯了怎么辦?
      A:同個訂單購買多個商品可能會分為一個以上包裹發出,可能不會同時送達,建議查看訂單詳情是否是 部分發貨狀態;如未收到,可聯系在線客服或者致電4000-520-616。

      Q:退換貨/維修需要多長時間?
      A:一般情況下,退貨處理周期為客戶收到產品一個月內(以快遞公司顯示簽收時間為準),包裝規格、 數量、品種不符,外觀毀損、短缺或缺陷,請在收到貨24小時內申請退換貨;特殊商品以合同條款為準。

    何為188

    極簡而嚴謹,我們僅銷售188款生物醫學科研用品,款款都是爆款;因為少所以聚焦,聚焦甄選每一款產品,聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知
    • 品質甄選,正品保證

    • 自營電商,廠家直采

    • 極簡主義,188精品

  • <menu id="uuusw"></menu>
  • <table id="uuusw"></table>
    上床视频