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    當前位置 ： Millipore >>> Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit
    Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit
    • Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

    Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

    價格: ￥7560.00 市場價: 12600.00
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    貨號: ECM340
    品牌: Millipore
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      • Description
        CatalogueNumberECM340
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionHumansVCAM-1ELISA
        OverviewVascularcelladhesionmolecule-1(VCAM-1,CD106)isamemberoftheimmunoglobulingenesuperfamily.TwoformsofVCAM-1withsixorsevenextracellularIg-likedomainsariseduetoalternativesplicingfromaseven-domainVCAM-1(7DVCAM-1).7DVCAM-1isthedominantformexpressedbyculturedhumanendothelialcells[Cybulskyetal.,1991;Hessionetal.,1991].Homologyofdomains1through3withdomains4through6suggestsageneduplicationevent[Cybulskyetal.,1991;Hessionetal.,1991].TheCDNAof7DVCAM-1predictsacoreproteinofapproximately81kDawithsevensitesforN-linkedglycosylation.Uponcompleteglycosylationthematureproteinhasamolecularweightofapproximately102kDa.Thisobservationisingeneralagreementwiththe110kDaproteinimmunoprecipitatedfromcytokine-activatedendothelium[Carlosetal.,1990;Riceetal.,1990].

        VCAM-1appearstohavebeenhighlyconservedthroughevolution.BothratandmouseVCAM-1arehighlyhomologousattheproteinleveltothehumanVCAM-1(77%and76%,respectively)[Hessionetal.,1992].

        VCAM-1supportstheadhesionoflymphocytes,monocytes,naturalkillercells,eosinophilsandbasophils,throughitsinteractionwithintegrina4b1(VLA-4).VCAM-1/VLA-4interactionmediatesfirmadherenceofcirculatingnon-neutrophilicleukocytestoendothelium[Shararetal.,1995].VCAM-1alsoparticipatesinleukocyteadhesionoutsideofthevasculature,mediatingprecursorlymphocyteadhesiontobonemarrowstromalcells[Ryanetal.1991]andBcellbindingtolymphnodefolliculardendriticcells[Feedmanetal.,1992].VCAM-1isnotconstitutivelyexpressedonendothelium,butcanbeup-regulatedinvitroinresponsetoLPS,TNF-a,andIL-1[Carlos&Harlan,1990;Osbornetal.,1989],aswellastointerferon-gandIL-4[Masinovskyetal.1990].VCAM-1isalsopresentontissuemacrophages,dendritecells,bonemarrowfibroblasts,myoblastsandmyotubes.

        AsolubleformofVCAM-1hasbeendescribed[DeMaeyer&DeMaeyer-Buignard,1995;Lobbetal.,1991],andsolubleVCAM-1hasbeenfoundinserum[Gearingetal.,1995].IncreasedlevelsofsolubleVCAM-1canbedetectedinseveraldiseasestates,includingcancer[Banksetal.,1993;Fortisetal.,1995;Gearing&Newman,1993;Laietal.,1995],autoimmunedisorders[Gearing&Newman,1993;Doreduffyetal.,1995;Gruschwitzetal.,1995;Masonetal.,1993;Mrowka&Sieberth,1995;Spronketal.,1994;Wellicomeetal.,1993],infections[Jakobsenetal.,1994]andinflammations[Gearing&Newman,1993;Mrowka&Sieberth,1995;Adamsetal.,1995;Limetal.,1995].

        TestPrinciple:

        Ananti-VCAM-1monoclonalantibodyisadsorbedontomicrowells.

        SolubleVCAM-1presentinasampleorstandardthenbindstoantibodiesadsorbedtothemicrowells.Amixtureofbiotin-conjugatedmonoclonalanti-VCAM-1antibodyandStreptavidin-HRPconjugateisadded.Biotinylatedanti-VCAM-1antibodybindstoVCAM-1capturedbythefirstantibody.

        Streptavidin-HRPbindstothebiotinylatedanti-VCAM-1.Unboundbiotinylatedanti-VCAM-1andStreptavidin-HRPconjugateisremovedwithawashstepandHRPsubstratesolutionisaddedtothewells.

        ThepairofmonoclonalantibodiesusedinthisELISAdetectthesolubleformofVCAM-1presentinserum,plasma,urine,andotherBIOLOGicalfluids.

        Anamountofcoloredproductisformed,proportionaltotheamountofsolubleVCAM-1presentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromsixsolubleVCAM-1standarddilutionsandtheVCAM-1sampleconcentrationisdetermined.

        Application:

        TheVCAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleVascularCellAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherbiologicalfluids.TheVCAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures.
        AlternateNames
        • CD106
        MaterialsRequiredbutNotDelivered-5mLand10mLgraduatedPipettes

        -5μLto1000μLadjustablesinglechannelmicropipetteswith

        disposabletips

        -50μLto300μLadjustablemultichannelmicropipettewithdisposabletips

        -Multichannelmicropipettereservoir

        -Beakers,flasks,cylindersnecessaryforpreparationofreagents

        -Devicefordeliveryofwashsolution(multichannelwashbottleor

        automaticwashsystem)

        -MicrowellstripreadercapableofreADIngat450nm(620nmas

        optionalreferencewavelength)

        -Glass-distilledordeionizedwater

        -Statisticalcalculatorwithprogramtoperformlinearregressionanalysis.
        ProductInformation
        Components
        • ·2aluminumpoucheswith3MicrowellStrips(16wellsperstrip)coatedwithMonoclonalAntibody(murine)tohumanVCAM-1
        • ·1vial(0.1mL)ConjugateMixturecontainingBiotin-ConjugatedAnti-VCAM-1Monoclonal(murine)AntibodymixedwithStreptavidin-HRP*
        • ·2vials(200ng/mLasreconstituted)solubleVCAM-1Standard,lyophilized*
        • ·1viallyophilizedcontrolserum
        • ·1bottle(50mL)WashBufferConcentrate20X(PBSwith1%Tween20)*
        • ·1vial(5mL)AssayBufferConcentrate20X(PBSwith1%Tween20and10%BSA)*
        • ·1vial(7mL)SubstrateSolutionI(tetramethyl-benzidine)
        • ·1vial(7mL)SubstrateSolutionII(0.02%bufferedhydrogenperoxide)
        • ·1vial(12mL)StopSolution(1MPhosphoricAcid,H3PO4),readytouse
        • ·1MicrowellStripHolder
        • ·2AdhesivePlateCovers
        • ·reagentscontaining0.01%thimerosalaspreservative
        DetectionmethodChromogenic
        StorageandShippingInformation
        StorageConditionsPrecautions:

        ·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures.

        ·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources.

        ·Donotusekitreagentsbeyondexpirationdateonlabel.

        ·Donotexposekitreagentstostronglightduringstorageorincubation.

        ·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens.

        ·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

        ·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal.

        ·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes.

        ·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents.

        ·Exposuretoacidswillinactivatetheconjugate.

        ·Glass-distilledwaterordeionizedwatermustbeusedforreagent

        preparation.

        ·Substratesolutionsmustbeatroomtemperaturepriortouse.

        ·Sinceexactconditionsmayvaryfromassaytoassay,astandard

        curvemustbeestablishedforeveryrun.

        ·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults.

        ·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse.

        ·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods.

        ·Anyhumananti-mouseIgGantibody(HAMA)presentmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent.
        Applications
        ApplicationThisHumansVCAM-1ELISAisusedtomeasure&quantifysVCAM-1levelsinInflammation&Immunology&Neuroscienceresearch.
        KeyApplications
        • ELISA
        BiologicalInformation
        SpeciesReactivity
        • Human
        AnalytesAvailable
        • sVCAM-1
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneisamemberoftheIgsuperfamilyandencodesacellsurfacesialoglycoproteinexpressedbycytokine-activatedendothelium.ThistypeImembraneproteinmediatesleukocyte-endothelialcelladhesionandsignaltransduction,andmayplayaroleinthedevelopmentofartherosclerosisandrheumatoidarthritis.Twoalternativelysplicedtranscriptsencodingdifferentisoformshavebeendescribedforthisgene.
        GeneSymbol
        • VCAM1
        • INCAM-100
        • MGC99561
        • CD106
        • DKFZp779G2333
        • L1CAM
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P19320#Importantincell-cellrecognition.Appearstofunctioninleukocyte-endothelialcelladhesion.Interactswiththebeta-1integrinVLA4onleukocytes,andmediatesbothadhesionandsignaltransduction.TheVCAM1/VLA4interactionmayplayapathophysiologicrolebothinimmuneresponsesandinleukocyteemigrationtositesofinflammation.
        SIZE:739aminoacids;81276Da
        SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
        TISSUESPECIFICITY:Expressedoninflamedvascularendothelium,aswellasonmacrophage-likeanddendriticcelltypesinbothnormalandinflamedtissue.
        DOMAIN:SwissProt:P19320EitherthefirstorthefourthIg-likeC2-typedomainisrequiredforVLA4-dependentcelladhesion.
        PTM:Sialoglycoprotein.
        DISEASE:SwissProt:P19320#Mayplayanimportantroleinthegenesisofatherosclerosisandrheumatoidarthritis.
        SIMILARITY:Contains7Ig-likeC2-type(immunoglobulin-like)domains.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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