• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： NEB >>> NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/500 reactions
    NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/500 reactions
    • NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/500 reactions

    NEB/NEBNext??Library Quant Kit for Illumina?/E7630S/500 reactions

    價格: ￥5040.00 市場價: 8400.00
    打印產品信息
    貨號: E7630S-500reactions
    品牌: NEB
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即購買 加入購物車

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題

      • Description:











        TypicalresultsfromtheNEBNextLibraryQuantKitonaBio-RadCFX96Touch(A)andanAppliedBiosystems7500Fastreal-timeqPCRinstrument(B).Amplificationcurvesareshownontheleftandresultingstandardcurvesontheright.Alldefaultsettingswereusedonbothplatforms.ABI7500Fastassayincluded1XROX(LowConcentration)andtheROXnormalization.
        Accuratequantitationofnext-generationsequencinglibrariesisessentialformaximizingdataoutputandqualityfromeachsequencingrun.ForIlluminasequencingspecifically,accuratequantitationoflibrariesiscriticaltoachieveoptimalclusterdensities,arequirementforoptimalsequenceperformance.qPCRisconsideredtobethemostaccurateandeffectivemethodoflibraryquantitation,providingconsiderablyhigherconsistencyandreproducibilitythanelectrophoresisorspectrophotometry,whichmeasuretotalnucleicacidconcentration.Amplification-basedmethodsquantitateonlythosemoleculesthatcontainbothadaptorsequences,therebyprovidingamoreaccurateestimateoftheconcentrationofthelibrarymoleculesthatcanbesequenced.

        TheNEBNextLibraryQuantKitdeliverssignificantimprovementstoqPCR-basedlibraryquantitationfornextgensequencing.TheNEBNextLibraryQuantKitforIlluminacontainscomponentsthatareoptimizedforqPCR-basedquantitationoflibrariespreparedforIlluminanext-generationsequencingplatforms.TheNEBNextLibraryQuantKitcontainsprimerswhichtargettheP5andP7Illuminaadaptorsequencesandasetofhigh-quality,pre-dilutedDNAstandardstoenablereliablequantitationofdilutedDNAlibrariesbetween150–1000bp.?

        Features
        • ProvidesmoreaccurateandreproducIBLequantvaluesthanalternativemethodsandkits
        • CompatiblewithlibrarieswithabroadrangeofinsertsizesandGCcontent,madebyavarietyofmethods
        • Requiresonly4standards,allowingmorelibrariestobequantitatedperkit?
        • Suppliedwithaconvenient,LibraryDilutionBuffer
        • TheNEBNextLibraryQuantMasterMixrequiresonlytheadditionofprimers
        • Utilizesasingleextensiontimeforalllibraries,regardlessofinsertsize
        • LibraryquantvaluescanbeeasilycalculatedusingNEB’sonlinetool,atNEBioCalculator.neb.com
        • ROXisincludedinthekit,forusewithqPCRinstrumentsthatrequireareferencedyefornormalization
        NEBNextLibraryQuantKitworkflow?NEBNext Library Quant Kit workflow
        qPCRprovidesmoreconsistentlibraryquantitationresultsthanBioanalyzer?analysis
        qPCR provides more consistent library quantitation results than Bioanalyzer? analysis

        Concentrationsof4librariesweredeterminedbytheNEBNextLibraryQuantKit(orange)andcomparedtovaluesmeasuredusingtheAglientBioanalyzer(blue).ComparedtoNEBNext’sqPCR-basedmethod,theBioanalyzerconcentrationsdisplayedagreaterlevelofvariation.

        GreaterreproducibilityoflibraryquantitationwiththeNEBNextLibraryQuantKit?
        Greater reproducibility of library quantitation with the NEBNext Library Quant Kit
        Three340–400bplibrarieswerequantitatedby4differentusers2–4timesusingeithertheNEBNextorKapaTMLibraryQuantificationKit(Universal).AnotableimprovementinquantitationconsistencywasobservedforconcentrationsdeterminedbytheNEBNextKit(orange)versusthosefromtheKapakit(gray).?
        Greaterlot-to-lotconsistencyofstandardswiththeNEBNextLibraryQuantKit?
        Greater lot-to-lot consistency of standards with the NEBNext Library Quant Kit
        AccurateqPCRquantitationrequirestheuseofhigh-qualityDNAstandardswithknownconcentrations.TheNEBNextLibraryQuantKitcontains4standardsproducedwithahighlevelofbothquantitationaccuracyandconsistency.Thisfigureshowsdatafrom>70totalrunsfrom4lotsofbothNEBNext(orange)andKapa(gray)standards,withallCqvaluesplotted.Boxandwhiskersindicatemeanandquartiles.TheNEBNextLibraryStandardsdisplayedmuchlowervariationinCq,resultinginmoreconsistentquantitationperformance.?
        TheNEBNextLibraryQuantKitvaluesenableoptimalclusterdensities
        The NEBNext Library Quant Kit values enable optimal cluster densities
        SevendifferentlibrarieswerequantitatedusingeithertheNEBNextLibraryQuantKit(orange)ortheKapaLibraryQuantificationKit(Universal)(gray).Undilutedlibraryconcentrationsrangedfrom2–200nM.Librariesweredilutedto8pMandloadedontoaMiSeq?instrument(v2chemistry;MCSv2.4.1.3).LibrariesquantitatedwiththeNEBNextkitresultedinarawclusterdensityaverageof1160k/mm2,directlyintheoptimalrangeof900–1300k/mm2.Incontrast,librariesloadedbasedontheKapaquantitationaveragedonly660k/mm2.?
        WiththeNEBNextLibraryQuantKit,optimalclusterdensityisachievedfromquantitatedlibrarieswithabroadrangeoflibrarysizeandGCcontent
        With the NEBNext Library Quant Kit, optimal cluster density is achieved from quantitated libraries with a broad range of library size and GC content
        Librariesof310–963bpfromtheindicatedsourceswerequantitatedusingtheNEBNextLibraryQuantKit,thendilutedto8pMandloadedontoaMiSeq(v2chemistry;MCSv2.4.1.3).Libraryconcentrationsrangedfrom7–120nM,andresultingrawclusterdensityforalllibrarieswas965–1300k/mm2(ave.=1199).OptimalclusterdensitywasachievedusingconcentrationsdeterminedbytheNEBNextLibraryQuantKitforalllibrarysizes.?
        AccurateLibraryQuantitationisachievedwithabroadrangeoflibrarysizeandGCcontent
        Accurate Library Quantitation is achieved with a broad range of library size and GC content
        AselectionoflibrariessuccessfullyquantitatedwiththeNEBNextLibraryQuantKit.Librariesareplottedbysize,rangingfromsmallerlibraries(sRNA,FFPE)at150–230bptolargestlibrariesat980bp.Variousinputsourceswereused,rangingfrom20-70%GCcontent,asindicatedbytextcolor.LibrarieswerepreparedusingNEBNext,IlluminaTruSeq?NanoandKapaHyperlibraryprepkits(datanotshown).NodependenceonsizeorGCcontentwasobservedinlibraryquantitationwhenusingNEBNext.

        LotControl
        ThelotsprovidedintheNEBNextLibraryQuantKitforIlluminaaremanagedseparatelyandarequalifiedbyfunctionaltesting.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaasdescribedoneachindividualcomponentpageinthemanual.

        FunctionallyValidated
        EachsetofreagentsisfunctionallyvalidatedtogetherthroughqPCR-basedlibraryquantitationassays.Reagentspassthefunctionaltestbyadheringtostringentcriteriasetforassayefficiencyandquantitationcycles(Cq)foreachDNAstandardandnotemplatecontrolreaction.

        Forlargervolumerequirements,customizedandbulkpackagingisavailablebypurchasingthroughtheOEM/BulksdepartmentatNEB.Pleasecontactnebsolutions@neb.comforfurtherinformation.

        ReagentsSupplied

        Thefollowingreagentsaresuppliedwiththisproduct:

        Storeat(°C)Concentration
        NEBNextLibraryQuantMasterMix-20
        NEBNextLibraryQuantPrimerMix-20
        NEBNextLibraryQuantDNAStandard1-20
        NEBNextLibraryQuantDNAStandard2-20
        NEBNextLibraryQuantDNAStandard3-20
        NEBNextLibraryQuantDNAStandard4-20
        ROX(Low)-20
        ROX(High)-20
        NEBNext?LibraryDilutionBuffer-2010X

        Notes:

        StorageofKitComponents:AlloftheindividualcomponentsoftheNEBNextLibraryQuantKitforIlluminaarestableat–20°Cfortwoyears.Whennotinuse,kitcomponentsshouldbestoredat–20°C.Allcomponentsofthekit,includingthecombinedNEBNextLibraryQuantMasterMixandPrimerMix,arestableforatleast30freeze/thaws.OncethePrimerMixhasbeenaddedtotheNEBNextLibraryQuantMasterMix(seeProtocolonpage6ofmanual),theresultingmixcanbestoredat–20°Cforsevenmonths.Forshort-termstorage,thismixmaybestoredat4°Cforuptosixweeks.Reactionscanbepreparedatroomtemperature,butunusedportionsofthekitreagentsshouldbekeptoniceafterthawingthemforuse.ForReagentsSupplied:Ifyouneeddilutionbufferinadditiontowhatisprovidedinthekit,itcanbepurchasedseparately.
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： Immunomedics產品 | PelFreez-Bio價格 | D-Orland產品代理 | maxmedlabs廠家 | ezbiosystems代理 | 螞蟻淘 | 細胞周期蛋白抗體

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频