dianova/Anti-CD31 (Ms,Sw) aus Ratte (SZ31) – unkonj. für Paraffingewebe aus Maus/500 μl/DIA-310

Reactivity:

Der Antik?rper-Klon SZ31 reagiert als erster Antik?rper spezifisch mit CD31 in standardm?ssig Formalin-fixierten Paraffin-eingebetteten (FFPE) Mausgeweben. Klon SZ31 zeigt keine Kreureaktion mit humanem CD31.

Die überragende Spezifit?t von Klon SZ31 wird in mehr als 175 Publikationen (CiteAb) best?tigt.

Background:

CD31, auch bekannt als PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1), wird auf der Oberfl?che embryonaler und adulter Endothelzellen konstitutiv exprimiert. Sowie auf Zelloberfl?chen von Monozyten, Neutrophilen, Thrombozyten und verschiedener Untergruppen der T-Zellen. Zudem konnte das Molekül auf h?matopoetischen Stammzellen aus dem Knochenmark und embryonalen Stammzellen nachgewiesen werden. Cluster of Differentiation 31 (CD31) ist ein integrales Membranglykoprotein (130 kDa), welches als Mitglied der Immunglobulin-Superfamilie an der Vermittlung der Zell-Zell-Adh?sion beteiligt ist. Die von CD31 vermittelte endotheliale Zell-Zell-Interaktion, spielt hierbei eine entscheidende Rolle in der Angiogenese. Das Adh?sionsmolekül gilt als einer der wichtigsten Marker in der humanen Angiogenese, Studien zufolge kann der Nachweis von CD31 auf ein Tumorrezidiv deuten. Pathophysiologische Untersuchungen von CD31 in Maus-Modellsystemen waren bisher nur begrenzt m?glich, weil Standard-FFPE Gewebe ausgeschlossen werden mussten. Der Ratten-Klon SZ31 (Anti-CD31) l?st diese Probleme. Unser monoklonaler Antik?rper erlaubt erstmals immunhistochemische Analysen von CD31 in Formalin-fixierten Paraffingeweben (FFPE) der Maus und überzeugt durch beeindruckende Qualit?t der mikroskopischen Aufnahmen.

Immunhistochemischer Nachweis von Maus-CD31 (PECAM-1) in Formalin-fixierten, Paraffin-eingebetteten Gewebeschnitten

Der monoklonale Antik?rper Klon SZ31 reagiert spezifisch mit Endothelzellen in Gef??en und Kapillaren von:

aorta

aortic origin

endocardium

small intestine

small intestine

colon

brain

lymph nodes

bone marrow

mesenterial vessels

kidney

pancreas

(Bilder mit freundlicher Genehmigung von Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin)

Lung

Muscle

Spinal

Liver

Adenocarcinoma

Adenocarcinoma

(Bilder mit freundlicher Genehmigung von Prof. Dr. H. Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin)

Western Blot-Analyse

Immunoblot mit Zellextrakten aus Mauslunge, J558L Zellen und m-Lend Zellen. Antik?rper-Klon SZ31 (DIA-310 1:500) wurde zusammen mit einem Ziege Anti-Ratte-HRP Sekund?rantik?rper (1:10.000) zum Nachweis von CD31 verwendet.

Spur:

1. J558 Zellen (CD31-), 25μg Lysat
2. Mauslunge, 25μg Lysat
3. blank
4. m-Lend Zellen (CD31+), 12.5μg Lysat

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