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    當前位置 ： Millipore >>> Millipore/DET-HP1000 | IHC Select? HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi
    Millipore/DET-HP1000 | IHC Select? HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi
    • Millipore/DET-HP1000 | IHC Select? HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi

    Millipore/DET-HP1000 | IHC Select? HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi

    價格: ￥6048.00 市場價: 10080.00
    打印產品信息
    貨號: DET-HP1000
    品牌: Millipore
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      • Description
        CatalogueNumberDET-HP1000
        Replaces2240
        BrandFamilyChemicon®
        TradeName
        • IHCSelect
        • Chemicon
        DescriptionIHCSelect?HRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagentandSA_HRP),Prediluted
        OverviewIntendedUse

        TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect?PredilutedPrimaryAntibodyreagents,whichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseprimaryantibodies.

        GeneralPurposeReagents

        PrincipleofProcedure:

        TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemusesbiotin-avidin-HRPcomplexedantibodiestodetectrabbitormouseIgGinthePrimaryAntibody.Theprimaryantibodybindstospecificantigenspresentinthespecimen.Anyexcessantibodyisremovedbywashing.Next,theSecondaryAntibodiesReagent(conjugatedtobiotin)isaddedandwillreactwiththeprimaryantibodypresentonthetissue.Unboundantibodyisremovedbywashing.ThesampleisthenbyincubatedwiththeStreptavidin-HRPsolution,whichbindstothebiotin-labeledsecondaryantibodypresentonthetissue.Unboundenzymeisremovedbywashing.Thechromogenicdevelopmentreagent,3,3"diaminobenzidine(DABSubstrate),isthenaddedandreactswiththeHRPattachedtotheHRP-streptavidin-biotin-antibodycomplex.TheHRPactivityonthechromogenicsubstrateresultsinthedepositofbrowntoblackinsolubleprecipitateatthoseantigenicsitescontainingthespecificepitopesrecognizedbytheprimaryantibody.Tissuesprocessedinavarietyoffixativesincludingneutralbufferedformalin,B-5,andBouin"smaybeused.

        SummaryandExplanation:

        Immunohistochemicaltechniqueshavebeeninusefortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19411.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9.

        Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.ThesecondaryantibodyisconjugatedwithbiotinandtheStrepavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacolouredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstain(counterstain)isthenusedtovisualizethetissuestructure.
        MaterialsRequiredbutNotDelivered·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect?PredilutedPrimaryAntibodies)

        ·WashBuffer

        ·Chromogen

        ·Counterstain

        ·EndogenousBlockingAgent

        ·Positiveandnegativetissuecontrolslides

        ·Positivelychargedmicroscopeslides

        ·Glasscoverslips(24x40mm,No.1)

        ·Distilledordeionizedwater

        ·Permanentmountingmedium,Eukitt*AmericanHistologyorequivalent

        ·3%laboratorygradehydrogenperoxide

        ·10.0%neutralbufferedformalin15

        ·Xylene,ACSgrade

        ·Ethanol,absolute(100%)

        ·Latex,rubberor100%nitrilegloves(xyleneresistant)

        ·Timer

        ·Fumehood

        ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses

        ·60°Cdryingoven
        ProductInformation
        DetectionmethodChromogenic
        StorageandShippingInformation
        StorageConditionsWhenstoredat2-8°C,theIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight.

        Precautions:

        ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults.

        *Donotmixreagentsfromdifferentlots.

        *Donotallowtheslidestodryatanytimeduringthestainingprocedure.

        *Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite.

        *DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCentreimmediately.

        ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations.

        ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater.

        ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.
        Applications
        ApplicationIHCSelectHRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagent&SA_HRP),PredilutedforImmunohistochemistry(Paraffin).
        KeyApplications
        • Immunohistochemistry(Paraffin)
        BiologicalInformation
        SpeciesReactivity
        • Mouse
        • Rabbit
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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