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UE-D4054◆DAPI和Hoechst 染料非常相似，該如何選用？DAPI是一種非常普通的藍色核復染熒光染料，能對固定和通透的細胞和組織的細胞核進行非常明亮的標記。遺憾的是，人們普遍認為它是介于半通透性到非通透性之間的染色劑，對活細胞的染色效果也不一致。Hoechst 33342染料是細胞通透性染料，與DAPI染色有相似的染色結合機制和熒光顏色；它是活細胞成像的首選，且對固定細胞的標記效果和DAPI一樣好。◆DAPI染色的工作液濃度一般為多少，要怎么稀釋？該試劑用于細胞染色時，需要根據具體的實驗目的確定工作液濃度，常用的濃度0.5-10μg/ml，直接用水稀釋即可。◆在細胞膜和細胞核雙染實驗中，細胞膜染色用DiI，細胞核染色用DAPI，細胞固定后，先染色DiI還是先染色DAPI？先用DiI對細胞膜進行染色，后用DAPI染核。◆DAPI和Hoechst可以用來示蹤細胞嗎？不可以，這些染料與DNA和RNA的結合會影響核酸的正常功能，擾亂轉錄和增殖。◆DAPI能否用來染色植物細胞的原生質體？可以，有文獻報道使用DAPI染色原生質體，但是原生質體比較脆弱，DAPI對原生質體的殺傷力可能比較大。◆該試劑可對酵母菌進行熒光標記嗎？有文獻用DAPI染酵母樣品，但我們目前沒有相關經驗，建議查看相關文獻資料進行參考。◆該試劑可以染活細胞嗎？DAPI染色時，細胞均需要固定操作，對于活細胞的染色效果較差。◆請問可以用于石蠟切片的免疫組化，進行染核嗎？可以的。
4.A Network Pharmacology Approach to Investigate the Anticancer Mechanism and Potential Active Ingredients of Rheum palmatum L. Against Lung Cancer via Induction of ApoptosisQing Zhang, Jia Liu, Ruolan Li, Rong Zhao, Mengmeng Zhang, Shujun Wei, Dong Ran, Wei Jin, Chunjie WuFrontiers in Pharmacology
5.Antiepileptic Effects of Cicadae Periostracum on Mice and Its Antiapoptotic Effects in H2O2-Stimulated PC12 Cells via Regulation of PI3K/Akt/Nrf2 Signaling PathwaysQing Zhang,Ruo-Lan Li,Ting Tao,Jia-Yi Sun,Jia Liu,Ting Zhang,Wei Peng,Chun-Jie WuVolume20216.SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagyXingyu Li,Peili Hou,Wenqing Ma,Xuefeng Wang,Hongmei Wang,Zhangping Yu,Huasong Chang,Tiecheng Wang,Song Jin,Xue Wang,Wenqi Wang,Yudong Zhao,Yong Zhao,Chunqing Xu,Xiaomei Ma,Yuwei Gao,Hongbin Hecellular & molecular immunology7.DHA alleviates diet-induced skeletal muscle fber remodeling via FTO/m6A/DDIT4/PGC1α signalingWei Chen,Yushi Chen,Ruifan Wu,Guanqun Guo,Youhua Liu,Botao Zeng,Xing Liao,Yizhen Wang,Xinxia WangBMC Biology8.Guanxin V alleviates acute myocardial infarction by restraining oxidative stress damage, apoptosis, and fibrosis through the TGF-β1 signalling pathwayBo Liang,Xiao-Xiao Zhang,Rui Li,Yong-Chun Zhu,Xiao-Jie Tian,Ning Gu Phytomedicine參考文獻：
1.Assay for nanogram quantities of DNA in cellular homogenates
應用方向：DNA含量測定2.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses應用方向：診斷細胞培養的支原體感染3.Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin
應用方向：流式細胞術中細胞核含量測定及染色體分選4.Loss of HSulf-1 up-regulates heparin-binding growth factor signaling in cancer 應用方向：評估細胞凋亡5.Direct measurement of femtogram amounts of DNA in cells and chloroplasts by quantitative microspectrofluorometry. 應用方向：免疫熒光法和原位雜交法檢測細胞核和細胞器DNA6.SMAD2 and SMAD7 Involvement in the post-translational regulation of Muc4 via the transforming growth factor-β and interferon-γ pathways in rat mammary epithelial cells 應用方向：免疫熒光法和原位雜交法檢測細胞核和細胞器DNA7.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses 應用方向：取代溴化乙錠對瓊脂糖凝膠中的DNA進行染色8.Comparison of ethidium bromide and 4", 6"-diamidino-2-phenylindole as quantitative fluorescent stains for DNA in agarose gels 應用方向：取代溴化乙錠對瓊脂糖凝膠中的DNA進行染色9.Localization of polyphosphates in Saccharomyces fragilis, as revealed by 4′, 6-diamidino-2-phenylindole fluorescence 應用方向：DAPI可與多磷酸鹽和其他聚陰離子，硫酸葡聚糖和SDS結合10.InflUEnce of S-adenosylmethionine on 4′, 6-diamidino-2-phenylindole dihydrochloride (DAPI)-induced fluorescence of polyphosphate in the yeast vacuole 應用方向：DAPI可與多磷酸鹽和其他聚陰離子，硫酸葡聚糖和SDS結合11.Fluorescent Complexes of DNA With DAPI 4",6-diamidine-2-phenyl indole.2HCl or DCI 4",6-dicarboxyamide-2-phenyl Indole 應用方向：DAPI可與多磷酸鹽和其他聚陰離子，硫酸葡聚糖和SDS結合品質甄選，正品保證
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