The antiserum was produced against synthesized Acetyl-peptide derived from human p53 around the Acetylation site of Lys372.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC 1:50~100 ELISA: 1:10000
Specificity
p53 (Acetyl-Lys372) Antibody detects endogenous levels of p53 protein only when acetylated at Lys372.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TP53
Database Links
Gene ID: 7157 Swiss-Prot #: P04637
OMIM #: 191170 Unigene #: Hs.437460/Hs.740601
Target Modification
Acetyl
Modification Sites
H:K372
Application Images
Image 1
Western blot analysis of lysate from Jurkat cells, using p53 (Acetyl-Lys372) Antibody.