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    當前位置 ： Millipore >>> Millipore/CYT420 | ChemiKine? Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit
    Millipore/CYT420 | <i>ChemiKine?</i> Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit
    • Millipore/CYT420 | <i>ChemiKine?</i> Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit

    Millipore/CYT420 | ChemiKine? Pigment Epithelium Derived Factor, Sandwich ELISA/CYT420/1 kit

    價格: ￥5244.00 市場價: 8740.00
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    貨號: CYT420
    品牌: Millipore
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      • Description
        CatalogueNumberCYT420
        BrandFamilyChemicon®
        TradeName
        • ChemiKine
        • Chemicon
        DescriptionChemiKine?PigmentEpitheliumDerivedFactor,SandwichELISA
        OverviewIntroduction

        PigmentEpithelium-DerivedFactor(PEDF)wasfirstidentifiedasafactorsecretedbyprimaryretinalpigmentepithelial(RPE)cellsthatpromotesthesurvivalofneuronsinvitroandinvivo.MolecularcharacterizationofPEDFshowsittobea50kDamemberoftheserpinfamilyofproteaseinhibitors1.AsecondmodeofactionofPEDFwasidentifiedwiththedemonstrationthatPEDFinducesapoptosisofmicrovascularendothelialcells,apropertythatmaintainstheavascularnatureoftheretina2.Asanumberofophthalmologicdisordersarecharacterizedbydysregulationoftheretinalinnervationandmicrovasculature,PEDFservesasanimportantregulatorofhumaneyedisease3.Inaddition,PEDFappearstohavemultifunctionalantitumoractivityinaneuroblastomamodel,asSchwanncell-derivedPEDFinducesadifferentiated,lessmalignantphenotypeinneuroblastomacells,promotesfurthergrowthandsurvivalofSchwanncells,andinhibitsangiogenesis4.

        ChemiKine?PigmentEpithelium-DerivedFactor(PEDF)kitisasandwichstripwell-typeenzymeimmunoassay(EIA),whichmeasuresPEDF.ThekitwillmeasurePEDFofvitreousfluidofhumanorigin.

        TestPrinciple

        WiththeChemiKine?PEDFassaysystem,amousemonoclonalantibodygeneratedagainsthumanPEDFiscoatedontoamicroplateandisusedtocapturePEDFfromasample.Asecond,biotinylatedPEDF-specificmousemonoclonalantibodydetectsthecapturedPEDF.Peroxidase-labeledstreptavidindetectsthebiotinylatedmousemonoclonalantibody.AfteradditionofthesubstrateandstopsolutiontheamountofPEDFisdetermined.ThestandardcurvedemonstratesadirectrelationshipbetweenOpticalDensity(OD)andPEDFconcentration:i.e.,thehighertheODthehigherthePEDFconcentrationinthesample.
        AlternateNames
        • PEDF
        MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes

        2.37oCincubator

        3.Platesealers

        4.Pipettors&tipscapableofaccuratelymeasuring10-1000mL

        5.GraduatedSEROlogicalpipettes

        6.96-wellmicroplatereaderwith450nmfilter

        7.Graphpaperformanualplottingofdata

        8.Polypropylenetesttubesforstandardandsampledilutions

        9.Mechanicalvortex

        10.One1or2litercontainer

        11.Urea
        ProductInformation
        Components
        • PEDFELISAPlate:(PartNo.61597)One96-WellImmunoplatePre-coatedwithMouseanti-HumanPEDFMonoclonalAntibody,sealedinafoilpouch.
        • WashBufferConcentrate:(PartNo.60245)One100mL(10X)bottleofConcentrate.
        • AssayDiluent:(PartNo.60240)One60mLbottle(ReadytoUse).
        • PEDFStandard(Human):(PartNo.61600)Onevialcontaining250ngrecombinantfulllengthhumanPEDF(Lyophilized).
        • BiotinylatedMouseanti-HumanPEDFMonoclonalAntibody:(PartNo.61598)One50μLvial.
        • Streptavidin,HRPconjugated:(PartNo.61599)One50μLvial.
        • TMB/ESolution:(PartNo.60096)One10mLbottleofaReadytoUsesolutionof3,3",5,5"-tetramethylbenzidineinaproprietarybufferwithenhancer.
        • StopSolution(PartNo.60193):One12mLbottleofanHClsolution(ReadytoUse).
        DetectionmethodColorimetric
        StorageandShippingInformation
        StorageConditionsMaintaintheunopenedkitat2-8°Cuntilexpirationdateindicatedonthelabel.Afteropeningthekitmaintaineachcomponentat2-8°Cuntilexpirationdateindicatedonthelabel.Afterreconstitution,maintainthePEDFStandardat-20oCforupto30days.Thestandardmaybefrozenandthawedthreetimeswithoutlossofimmunoreactivity.Donotstorereconstitutedstandardat4oC,aslossofimmunoreactivitywillresult.

        Precautions

        ·WashBufferandAssayDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

        ·Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata.
        Applications
        ApplicationThisChemiKinePigmentEpitheliumDerivedFactor,SandwichELISAisusedtomeasure&quantifyPEDFlevelsinNeuroscienceresearch.
        KeyApplications
        • ELISA
        ApplicationNotesChemiKine?PEDFkitisdesignedtomeasuretheamountofPEDFincellculturesupernatants,tissuehomogenatesandBIOLOGicalfluid(vitreous,serum,plasma,andserum-free)samplesofhumanorigin.Thereareenoughreagentsincludedinthiskitforone96-wellimmunoassayplate.Runningduplicatewellsforsamplesandstandardsisrecommended.

        ForResearchUseOnly.Notforuseindiagnosticprocedures.
        BiologicalInformation
        HostMouse
        SpeciesReactivity
        • Human
        AntibodyTypeMonoclonalAntibody
        AnalytesAvailable
        • PEDF
        EntrezGeneNumber
        GeneSymbol
        • SERPINF1
        • Serpin-F1
        • PEDF
        • PIG35
        • EPC-1
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P36955#Neurotrophicprotein;inducesextensiveneuronaldifferentiationinretinoblastomacells.Potentinhibitorofangiogenesis.AsitdoesnotundergotheS(stressed)toR(relaxed)conformationaltransitioncharacteristicofactiveserpins,itexhibitsnoserineproteaseinhibitoryactivity.
        SIZE:418aminoacids;46342Da
        SUBCELLULARLOCATION:Secreted.Melanosome.Note=EnrichedinstageImelanosomes.
        TISSUESPECIFICITY:Retinalpigmentepithelialcellsandbloodplasma.
        DEVELOPMENTALSTAGE:Expressedinquiescentcells.
        DOMAIN:SwissProt:P36955TheN-terminal(AA44-121)exhibitsneuriteoutgrowth-inducingactivity.TheC-terminalexposedloop(AA382-418)isessentialforserpinactivity.
        PTM:TheN-terminusisblocked.
        SIMILARITY:Belongstotheserpinfamily.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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