| CatalogueNumber | CC117 |
| BrandFamily | Chemicon®
|
| TradeName | |
| Description | ChickenExtracellularChondroitinSulfateProteoglycans |
| Overview | DESCRIPTION/COMPOSITION:CC117containsamixtureoflarge,extracellularchondroitinsulfateproteoglycansisolatedfromembryonicchickenbrain.Aminoacidsequenceanalysesandimmunologicalstudieshavedemonstratedthatthemajorcomponentsofthismixtureareneurocan,phosphacan,versican,andaggrecan.Followingchondroitinasetreatmenttoremovetheglycosaminoglycanportionoftheseproteoglycans,themajorcomponentsofthispreparationresolvedonSDSgelsare250kDacoreproteinsrepresentingneurocanand400kDacoreproteinsrepresentingaggrecan,phosphacan,andversican.
*Quantity:Proteinasdeterminedusingthebicinchoninicacid(BCA)assay.Notethat100μgisameasurementofcorepolypeptides.Componentsofthismixturecontaincorepolypeptideplusattachedglycosaminoglycans |
| BackgroundInformation | Aggrecan,versican,andneurocanaremembersofthefamilyofhyaluronicacid-bindingproteoglycansandthereforeeachcontainshyaluronicacidbindingregions,Ig-likedomains,EGFlikedomains,lectin-likedomains,andcomplementregulatory-likedomains.Phosphacanisdistinct;itcontainsaregionofsimilaritytocarbonicanhydrase,afibronectintypeIIIdomain,andisasplicevariantofthetransmembraneproteinreceptortyrosinephosphataseequivalenttoitsextracellularportion.
AlthoughaggrecanwastrADItionallythoughtofasacartilageproteoglycanandneurocanandphosphacanwerethoughtofasneuralproteoglycans,recentstudiesindicatethatallfouroftheseproteoglycanshavebroadyetdistinctivedistributionpatternsinearlyembryos.Thepresenceoftheseproteoglycanshasnotbeencarefullyexaminedinadulttissuebeyondtheexpectedsites.Theseproteoglycansareanti-adhesionmoleculesandarecapableofinhibitingbothcell-ECMandcell-celladhesion. |