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    當前位置 : Millipore >>> Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg
    Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg
    • Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg

    Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg

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    貨號: CBL150
    品牌: Millipore
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      • Description
        CatalogueNumberCBL150
        Replaces04-460
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-CD3Antibody,cloneUCHT1
        AlternateNames
        • TCR
        ProductInformation
        FormatPurified
        PresentationThemonoclonalispresentedataconcentrationof100μg/1mlinphosphatebufferedsalinecontaining10mMsodiumazideand1mg/mlbovineserumalbumin.Werecommendthateachlaboratorydetermineanoptimumworkingtitreforuseinitsparticularapplication.
        StorageandShippingInformation
        StorageConditionsForusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C.
        Applications
        ApplicationAnti-CD3Antibody,cloneUCHT1detectslevelofCD3&hasbeenpublished&validatedforuseinFC,IF,IP,FUNC,IH.
        KeyApplications
        • FlowCytometry
        • Immunofluorescence
        • Immunoprecipitation
        • AffectsFunction
        • Immunohistochemistry
        ApplicationNotesIdentificationofCD3positiveTlymphocytesbyindirectimmunofluorescencestaining

        Leukaemiaandlymphomaphenotyping

        IdentificationofnormalandmalignantTlymphocytesinfrozentissuesections

        ImmunoprecipitationoftheCD3antigen

        MitogenicstimulationofTlymphocyteswithorwithoutthepresenceofIL-2

        Optimalworkingdilutionsmustbedeterminedbytheenduser.
        BIOLOGicalInformation
        ImmunogenPurifiedhumanTcells
        CloneUCHT1
        HostMouse
        SpecificityThisantibodyrecognizes22/26/30kDamolecularweightlymphocytesurfacemoleculesassociatedwiththeTcellantigenreceptorcomplex.CD3iscloselyassociatedonthelymphocytecellsurfacewiththeTcellantigenreceptorTcR(2-4).ItisbelievedthattheCD3complextransmitsactivationalsignalstotheTcell"sinterioruponantigenrecognitionbyTcR.CD45(LCA),aproteintyrosinephosphatase,isapotentregulatorofsignaltransductionbytheCD3complex.ItreactswiththemajorityofperipheralbloodTlymphocytes,amajorproportionofthymocytes,themajorityofTcellchroniclymphocyticleukaemiacells,Sézaryleukaemiasandapproximately70%ofacutelymphoblasticleukaemiasofTcellorigin.
        Antigendistribution:
        Tcells100%
        PeripheralBloodlymphocytes75+7%
        Thymocytes68+5%
        Granulocytes<>
        Monocytes<>
        Bcells(E-)<>
        NKcells(CD16+)<>
        KNOWNSPECIESCROSSREACTIVITY:Negativewithrhesusmonkeyperipheralbloodleucocytesinflowcytometry
        FUSIONPARTNER:P3x63Ag8.653myelomacellline
        IsotypeIgG1
        SpeciesReactivity
        • Human
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryTheproteinencodedbythisgeneistheCD3-epsilonpolypeptide,whichtogetherwithCD3-gamma,-deltaand-zeta,andtheT-cellreceptoralpha/betaandgamma/deltaheterodimers,formstheT-cellreceptor-CD3complex.Thiscomplexplaysanimportantroleincouplingantigenrecognitiontoseveralintracellularsignal-transductionpathways.Thegenesencodingtheepsilon,gammaanddeltapolypeptidesarelocatedinthesameclusteronchromosome11.TheepsilonpolypeptideplaysanessentialroleinT-celldevelopment.Defectsinthisgenecauseimmunodeficiency.ThisgenehasalsobeenlinkedtoasusceptibilitytotypeIdiabetesinwomen.
        GeneSymbol
        • CD3E
        • T3E
        • TCRE
        Non-ReactiveSpecies
        • Monkey
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P07766#TheCD3complexmediatessignaltransduction.
        SIZE:207aminoacids;23147Da
        SUBUNIT:TheTCR/CD3complexofT-lymphocytesconsistsofeitheraTCRalpha/betaorTCRgamma/deltaheterodimercoexpressedatthecellsurfacewiththeinvariantsubunitsofCD3labeledgamma,delta,epsilon,zeta,andeta.
        SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
        SIMILARITY:SwissProt:P07766##Contains1Ig-like(immunoglobulin-like)domain.&Contains1ITAMdomain.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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