The antiserum was produced against synthesized peptide derived from human nNOS.
Immunogen Range
818-867
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
nNOS Antibody detects endogenous levels of total nNOS protein.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NOS1
Database Links
Gene ID: 4842 Swiss-Prot #: P29475
OMIM #: 163731 Unigene #: Hs.654410
Target Modification
Unmodified/Total
Modification Sites
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using nNOS Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from Raw264.7 cells, treated with INF 2500u/ml 10', using nNOS Antibody. The lane on the right is blocked with the synthesized peptide.